AB302958

Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(59 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat, Transfected cell line - Human samples. Cited in 59 publications.

대체 명칭 보기

PI3-kinase subunit gamma, PI3K-gamma, PI3Kgamma, PtdIns-3-kinase subunit gamma, Phosphoinositide-3-kinase catalytic gamma polypeptide, Serine/threonine protein kinase PIK3CG, p120-PI3K, PtdIns-3-kinase subunit p110-gamma, p110gamma, PIK3CG

6 이미지

Supplier Data

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/2000 (0.251 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Postive staining on (A) HEK-293T cells transfected with a PIK3CG expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab302958 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 (5.02 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Postive staining on human breast carcinoma. The section was incubated with ab302958 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 (5.02 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Postive staining on human spleen. The section was incubated with ab302958 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 (5.02 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human tonsil. The section was incubated with ab302958 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 (5.02 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat spleen. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 (5.02 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Postive staining on mouse spleen. The section was incubated with ab302958 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25156-60

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase catalytic subunit gamma also known as PI3K-gamma is an enzyme important in cellular signaling. PI3K-gamma is part of the PI3K family which includes different classes of signal transducing enzymes. The catalytic subunit with a molecular weight of approximately 110 kDa becomes activated primarily in response to activation of G protein-coupled receptors. It is abundantly expressed in hematopoietic cells but also found in other tissues like the heart and lungs indicating its significant role in diverse cellular contexts. The enzyme functions by phosphorylating phosphoinositides an important step in initiating various downstream signaling pathways.

Biological function summary

PI3K-gamma plays an important role in mediating immune responses. It is a part of a larger dimeric complex coupling with a regulatory subunit to modulate its activity and specificity. This complex formation is vital in regulating leukocyte chemotaxis endothelial cell migration and cardiac function. Signaling through PI3K-gamma influences the activation and migration of immune cells aiding in the body's defense mechanism against infections and inflammation. Additionally the protein regulates heart contractility contributing to cardiac physiology.

Pathways

PI3K-gamma is an essential component in the PI3K-Akt signaling pathway. This pathway modulates various cellular processes including cell proliferation survival and metabolism. Reports link PI3K-gamma to the regulation of mTOR pathway which further affects protein synthesis and autophagy. Within these pathways it aligns with proteins such as Akt and mTORC1 facilitating signals that control cell growth and resistance to apoptosis. These pathways demonstrate the enzyme's involvement in maintenance of cellular homeostasis and adaptability.

PI3K-gamma has been implicated in inflammatory diseases and cardiovascular disorders. Enhanced PI3K-gamma activity often correlates with hyperactive immune responses contributing to conditions like rheumatoid arthritis and asthma. Its aberrant signaling also associates with heart failure due to its regulatory role in cardiac myocyte function. In the context of these diseases there is interaction with proteins such as NF-kB and Bcl-2 which get involved in pro-inflammatory signaling and cell survival respectively. The regulation of PI3K-gamma offers potential therapeutic targets for managing these conditions effectively.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation, activation, and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity : both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway downstream of RASGRP4-mediated Ras-activation, to promote neutrophil functional responses (By similarity).

See full target information PIK3CG

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제품이 사용된 논문 (59)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Theranostics 15:9580-9600 PubMed41041054

2025

Macrophage-TREM2 promotes cardiac repair by restricting the infiltration of CD8 T cells via CXCL16-CXCR6 axis after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhang,Sheng Wang,Yu Ding,Tao Zheng,Jie Sheng,Yanshan Chen,Zhiyue Wang,Ximei Dai,Canbiao Wang,Long Ma,Jing Pan,Yunming Zhang,Longjiang Zhang

Global spine journal :21925682251383489 PubMed41001708

2025

Intervention of Rutin and Ochnaflavone in the Attenuation of Neuroinflammation and Neuronal Apoptosis in Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yu,Xuhao Yang,Anyuan Dai,Zhihe Yun,Wu Xue,Tianyang Yuan,Xinyu Nie,Inbo Han,Yanting Liu,A O Wang,Qinyi Liu

Physiological reports 13:e70553 PubMed40969143

2025

Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Xu,Wenli Li,Jun Shi,Yanfang Guo

Journal of clinical and translational hepatology 13:555-567 PubMed40937080

2025

CCNE1 Promotes the Progression of Hepatic Precancerous Lesion and the Malignant Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xue Hu,Lichao Yao,Wenzhi Guo

Frontiers in pharmacology 16:1600435 PubMed40918528

2025

Gypenoside XLIX inhibiting PI3K/AKT/FOXO1 signaling pathway mediated neuronal mitochondrial autophagy to improve patients with ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yonglei Liu,Hongdie Mao,Zhengguang Sha,Jishuai Zhao,Hui Cai,Rong Xi,Zhenzhu Zhao,Xiaoling Yin,Lin Yang,Changyun Liu

World journal of gastrointestinal oncology 17:105321 PubMed40837760

2025

LncRNA SNHG5 modulates cell proliferation and migration through the miR-92a-3p/BTG2 axis in gastric cancer by the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Qi Mao,Mei-Lin Zhang,Liang Zhong,Xu-Dong Xu,Xin-Hai Wang,Du-Yi Pan,Fu-Sheng Zhou,Jia-Xin Huang,Xian-Guang Zhao,Jia-Jie Chen,Xiao-Yun Jiang,Xu Sun,Wei-Qun Ding

Scientific reports 15:27739 PubMed40730854

2025

CIAPIN1 promotes survival, proliferation, migration and glycolysis of endometrial cancer cells through PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huanmei Sun,Juan Shao,Yingchun Duan,Jiashi Gu

Iranian journal of basic medical sciences 28:946-954 PubMed40703755

2025

Schisandrin B regulates the SIRT1/PI3K/Akt signaling pathway to ameliorate Ang II-infused cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Zhang,Mengqing Shi,Zhongying Xing,Jie Su,Yuying Gu,Zhongping Ning

American journal of translational research 17:4689-4700 PubMed40672621

2025

Undemineralized dentin matrix particles accelerate blood vessel formation in a critical-sized skull defect through activating the TGF-β/PI3K signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zu,Xiangwen Zhou,Qingsong Jiang

Journal of cellular and molecular medicine 29:e70711 PubMed40665565

2025

SERPINB6 Promotes Epithelial-Mesenchymal Transition via PI3K/AKT/mTOR Signalling Pathway in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Wang,Haiyang Wang,Wenhao Zheng,Heng Wang,Wenhua Yu,Quan Du

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60]

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

Immunohistochemistry - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (AB302958)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (59)

Product promise