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AB32401

Anti-PI 3 Kinase p110 delta antibody [Y387]

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(13 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PI 3 Kinase p110 delta antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 13 publications.
3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [Y387] (AB32401)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [Y387] (AB32401)

Immunofluorescent staining of HeLa cells using ab32401 at 1/250 dilution.

Western blot - Anti-PI 3 Kinase p110 delta antibody [Y387] (AB32401)
  • WB

Lab

Western blot - Anti-PI 3 Kinase p110 delta antibody [Y387] (AB32401)

Western blot : Anti-PI 3 Kinase p110 delta antibody [Y387] ab32401 staining at 1/500 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 120 kDa in Wild-type PC-3 cell lysates with no signal observed at this size in PIK3CD knockout PC-3 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401) at 1/500 dilution

Lane 1:

Wild-type PC-3 at 20 µg

Lane 2:

PIK3CD knockout PC-3 at 20 µg

Lane 3:

Jurkat at 20 µg

Lane 4:

K562 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 120 kDa

Observed band size: 120 kDa

false

Western blot - Anti-PI 3 Kinase p110 delta antibody [Y387] (AB32401)
  • WB

Unknown

Western blot - Anti-PI 3 Kinase p110 delta antibody [Y387] (AB32401)

All lanes:

Western blot - Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401) at 1/500 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 119 kDa

Observed band size: 110 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-PI 3 Kinase p110 delta antibody [Y387] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y387

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32401 recognises PI3-Kinases (PI3-Ks).

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대체품

Primary Antibodies

AB200372

Anti-PI 3 Kinase p110 delta antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p110 delta antibody (AB200372)

  • 0
  • 0
Primary Antibodies

AB109006

Anti-PI 3 Kinase p110 delta antibody [EPR386]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)

  • 0
  • 0
Primary Antibodies

AB236049

Anti-PI 3 Kinase p110 delta antibody [EPR386] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] - BSA and Azide free (AB236049)

  • 0
  • 0

보완 제품

Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2
Primary Antibodies

AB140307

Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (AB140307)

  • 0
  • 0
Primary Antibodies

AB179463

Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB179463)

  • 0
  • 0
Primary Antibodies

AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

20ul selling size|Recombinant

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoinositide 3-kinase p110 delta also known as PI3Kδ or PIK3CD is a catalytic subunit of the class I PI3K enzyme complex. It plays a significant role in the phosphorylation of the 3'-hydroxyl group of inositol lipids which acts as a signal transducer. The molecular weight of p110 delta is approximately 110 kDa. This protein is highly expressed in leukocytes especially in B and T lymphocytes. Expression can also be seen in other cell types like neutrophils.
Biological function summary

P110 delta is involved in immune cell signaling as it is part of the class I PI3K complex. PI3Kδ functions in conjunction with p85 regulatory subunits to modulate various intracellular processes. The complex plays an important role in regulating cell growth proliferation and survival by mediating signals from growth factors or cytokines. It is also critical in the development and function of the adaptive immune system.

Pathways

PI3Kδ participates in the PI3K/AKT signaling pathway an important regulatory pathway in cell growth and survival. Another significant pathway involving p110 delta is the B-cell receptor (BCR) signaling pathway which is important for B cell development. Within these pathways PI3Kδ interacts with proteins such as AKT mTOR and BCR-related proteins that propagate downstream signaling events.

Aberrant activity of PI3Kδ has been linked to various immune-related diseases such as chronic lymphocytic leukemia (CLL) and rheumatoid arthritis. The overactivation of PI3Kδ in CLL correlates with enhanced survival and proliferation of malignant B cells. Additionally PI3Kδ dysfunction can influence inflammation pathways contributing to autoimmune conditions. The protein often works in concert with other proteins in these disease contexts such as B cells in CLL where it affects signaling via the B-cell receptor pathway.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 9235916). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Plays a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Plays important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity.
See full target information PIK3CD

대체 명칭 보기

PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD
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제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 5:740 PubMed35879421

2022

PI3Kδ activity controls plasticity and discriminates between EMT and stemness based on distinct TGFβ signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Agnetti,Vanessa Bou Malham,Christophe Desterke,Nassima Benzoubir,Juan Peng,Sophie Jacques,Souad Rahmouni,Emanuel Di Valentin,Tuan Zea Tan,Didier Samuel,Jean Paul Thiery,Ama Gassama-Diagne

Nature 542:489-493 PubMed28199309

2017

Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mara Compagno,Qi Wang,Chiara Pighi,Taek-Chin Cheong,Fei-Long Meng,Teresa Poggio,Leng-Siew Yeap,Elif Karaca,Rafael B Blasco,Fernanda Langellotto,Chiara Ambrogio,Claudia Voena,Adrian Wiestner,Siddha N Kasar,Jennifer R Brown,Jing Sun,Catherine J Wu,Monica Gostissa,Frederick W Alt,Roberto Chiarle

Respiratory research 17:145 PubMed27825347

2016

Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients.

Applications

IP

Species

Human

Javier Milara,Angela Cervera,Alfredo de Diego,Celia Sanz,Gustavo Juan,Amadeu Gavaldà,Montserrat Miralpeix,Esteban Morcillo,Julio Cortijo

Frontiers in physiology 7:476 PubMed27826249

2016

Cannabinoid CB Receptors Are Localized in Striated Muscle Mitochondria and Regulate Mitochondrial Respiration.

Applications

IP

Species

Mouse

Juan Mendizabal-Zubiaga,Su Melser,Giovanni Bénard,Almudena Ramos,Leire Reguero,Sergio Arrabal,Izaskun Elezgarai,Inmaculada Gerrikagoitia,Juan Suarez,Fernando Rodríguez De Fonseca,Nagore Puente,Giovanni Marsicano,Pedro Grandes

Oncogene 35:2801-12 PubMed26364609

2015

Ceramide limits phosphatidylinositol-3-kinase C2β-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid.

Applications

Unspecified application

Species

Unspecified reactive species

K Kitatani,T Usui,S K Sriraman,M Toyoshima,M Ishibashi,S Shigeta,S Nagase,M Sakamoto,H Ogiso,T Okazaki,Y A Hannun,V P Torchilin,N Yaegashi

Nature communications 6:5937 PubMed25583025

2015

Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Peng,Aline Awad,Sokhavuth Sar,Ola Hamze Komaiha,Romina Moyano,Amel Rayal,Didier Samuel,Annette Shewan,Bart Vanhaesebroeck,Keith Mostov,Ama Gassama-Diagne

The Journal of allergy and clinical immunology 134:314-22 PubMed24636089

2014

Roflumilast N-oxide reverses corticosteroid resistance in neutrophils from patients with chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Milara,Javier Lluch,Patricia Almudever,Jose Freire,Qian Xiaozhong,Julio Cortijo

Laboratory investigation; a journal of technical m 93:566-76 PubMed23439433

2013

PI3K p110γ overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition.

Applications

WB

Species

Human

Enrico Conte,Elisa Gili,Mary Fruciano,Martina Korfei,Evelina Fagone,Maria Iemmolo,Debora Lo Furno,Rosario Giuffrida,Nunzio Crimi,Andreas Guenther,Carlo Vancheri

Proceedings of the National Academy of Sciences of 109:12165-70 PubMed22689948

2012

Neuregulin 1-ErbB4-PI3K signaling in schizophrenia and phosphoinositide 3-kinase-p110δ inhibition as a potential therapeutic strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Law,Yanhong Wang,Yoshitatsu Sei,Patricio O'Donnell,Patrick Piantadosi,Francesco Papaleo,Richard E Straub,Wenwei Huang,Craig J Thomas,Radhakrishna Vakkalanka,Aaron D Besterman,Barbara K Lipska,Thomas M Hyde,Paul J Harrison,Joel E Kleinman,Daniel R Weinberger

Journal of immunology (Baltimore, Md. : 1950) 187:2376-85 PubMed21810603

2011

Enhanced phosphoinositide 3-kinase δ activity is a frequent event in systemic lupus erythematosus that confers resistance to activation-induced T cell death.

Applications

IP

Species

Unspecified reactive species

Abel Suárez-Fueyo,Domingo F Barber,Jorge Martínez-Ara,Antonio C Zea-Mendoza,Ana C Carrera
제품이 사용된 논문 모두 보기
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