Anti-PI 3 Kinase p55 gamma antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p55 gamma antibody (AB235234)

100% methanol-fixed, 0.3% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PI 3 Kinase p55 gamma (Green) using ab235234 at 15 μg/ml overnight at 4°C. The secondary antibody was Donkey anti-Goat IgG DiLight™ 488 Conjugated antibody at 15 μg/ml for 1 hour at room temperature. The nuclear counterstain was DAPI (Blue).

(A) DAPI

(B) ab235234 + Secondary

(C) Merge A+B

(D) Secondary only

Expected localization : Cytosol (UniProt), Nucleoplams (HPA) – distinct punctate staining in nucleus could indicate nucleoplasm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (AB235234)

Paraffin-embedded human tonsil tissue stained for PI 3 Kinase p55 gamma using ab235234 at 1/100 dilution for 30 minutes at room temperature, in immunohistochemical analysis. Secondary antibody was Donkey anti-Goat (HRP) 4 μg/ml for 20 minutes at room temperature. Counterstained with hematoxylin.

HIER using Citrate Buffer for 20min.

Panel A) Human tonsil H&E stain.
Panel B) ab235234 staining at 20X.
Panel C) ab235234 staining at 40X.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (AB235234)

Paraffin-embedded human tonsil tissue stained for PI 3 Kinase p55 gamma using ab235234 at 1/100 dilution for 30 minutes at room temperature, in immunohistochemical analysis. Secondary antibody was Donkey anti-Goat (HRP) (Ready to use). Counterstained with hematoxylin.

HIER using Citrate Buffer for 20min.

Panel A) Human testis H&E stain.
Panel B) ab235234 staining at 20X.
Panel C) ab235234 staining at 40X.

• WB

Supplier Data

Western blot - Anti-PI 3 Kinase p55 gamma antibody (AB235234)

All lanes:

Western blot - Anti-PI 3 Kinase p55 gamma antibody (ab235234) at 1/1000 dilution

All lanes:

Mouse testis lysate

Secondary

All lanes:

Goat IgG-HRP at 1/5000 dilution

Predicted band size: 54 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-PI 3 Kinase p55 gamma antibody (AB235234)

All lanes:

Western blot - Anti-PI 3 Kinase p55 gamma antibody (ab235234) at 1/1000 dilution

Lane 1:

PI 3 Kinase P55 gamma overexpressing HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

Empty vector HEK-293T lysate

Secondary

All lanes:

Goat IgG HRP at 1/40000 dilution

Predicted band size: 54 kDa

false

Exposure time: 75s