Anti-PI 3 Kinase p85 beta antibody [RP23040082]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 beta antibody [RP23040082] (AB313427)

Immunofluorescent analysis of 70% confluent log phase U-87 MG cells fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature labeling PI3K with ab313427 at 2-4 μg/mL, followed by Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a/ green). Nuclei (Panel b/ blue) were stained with DAPI. F-actin (Panel c/ red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic and membrane localization of PI3K. Panel e shows no primary antibody. The images were captured at 20X magnification.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 beta antibody [RP23040082] (AB313427)

Immunofluorescent analysis of HeLa cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling PI3 kinase p85 with ab313427, followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of PI3K.

• WB

Supplier Data

Western blot - Anti-PI 3 Kinase p85 beta antibody [RP23040082] (AB313427)

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta antibody [RP23040082] (ab313427) at 2 µg/mL

Lane 1:

Jurkat whole cell lysate

Lane 2:

NIH-3T3 whole cell lysate

Lane 3:

HEK293 whole cell lysate

Lane 4:

HeLa whole cell lysate

Lane 5:

MCF-7 whole cell lysate

Lane 6:

K562 whole cell lysate

Observed band size: 85 kDa

true

• WB

Supplier Data

Western blot - Anti-PI 3 Kinase p85 beta antibody [RP23040082] (AB313427)

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta antibody [RP23040082] (ab313427) at 0.5 µg/mL

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Lane 5:

NIH/3T3 cell lysate at 20 µg

Lane 6:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG-HRP Secondary Antibody at 1/5000 dilution

Predicted band size: 81.545 kDa

Observed band size: 80 kDa

true