AB226396

Anti-PICOT antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal PICOT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GLRX3 aa 250 to C-terminus.

대체 명칭 보기

PICOT, TXNL2, HUSSY-22, GLRX3, Glutaredoxin-3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2, PKCq-interacting protein

2 이미지

Supplier Data

Immunoprecipitation - Anti-PICOT antibody (AB226396)

PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 μg/ml.

Lane 1 : ab226396 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PICOT antibody (ab226396)

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-PICOT antibody (AB226396)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PICOT antibody (ab226396) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GLRX3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O76003

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab226396 was affinity purified using an epitope specific to PICOT immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PICOT also known as thioredoxin-interacting protein (TXNIP) or Vitamin D3 up-regulated protein 1 (VDUP1) is a protein with a mass of approximately 39 kDa. It interacts with several cellular components including thioredoxin acting as a regulatory inhibitor. PICOT is expressed in a variety of tissues with notable presence in heart and muscle tissues. It influences the redox state of the cell playing a significant role in oxidative stress response.

Biological function summary

PICOT acts as a regulator of the thiol redox system in cells. It forms a complex with thioredoxin thereby inhibiting its activity which leads to an accumulation of oxidized proteins. This activity makes PICOT an important player in redox signaling affecting cellular responses to oxidative stress. Through its interactions PICOT contributes to cellular processes such as apoptosis differentiation and proliferation mediated by redox changes.

Pathways

PICOT is integral to the cellular oxidative stress response and redox regulation pathways. It interacts with proteins like thioredoxin and possibly influences NADPH oxidase activity. By modulating thioredoxin PICOT impacts the NF-kB signaling pathway which plays a role in inflammation and immune responses. These pathways highlight PICOT's involvement in maintaining cellular homeostasis especially in stress conditions.

PICOT has links to diabetes and cardiovascular disorders. It affects glucose metabolism and insulin signaling connecting to proteins such as insulin receptor substrates. PICOT also involves in heart disorders given its role in cardiac muscle stress responses and connection to proteins like troponin. Understanding these relationships can aid in developing therapeutic approaches for managing oxidative stress-related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed : 26613676, PubMed : 27519415). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). Required for hemoglobin maturation (PubMed : 23615448). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.

See full target information GLRX3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells 12: PubMed37887353

2023

SLC38A5 Modulates Ferroptosis to Overcome Gemcitabine Resistance in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Myeong Jin Kim,Hyung Sun Kim,Hyeon Woong Kang,Da Eun Lee,Woosol Chris Hong,Ju Hyun Kim,Minsoo Kim,Jae-Ho Cheong,Hyo Jung Kim,Joon Seong Park

Scientific reports 11:21520 PubMed34728688

2021

Genetic variants in histone modification regions are associated with the prognosis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Gyoung Kang,Yong Hoon Lee,Shin Yup Lee,Jin Eun Choi,Sook Kyung Do,Mi Jeong Hong,Jang Hyuck Lee,Ji Yun Jeong,Young Woo Do,Eung Bae Lee,Kyung Min Shin,Won Kee Lee,Sun Ha Choi,Hye Won Seo,Seung Soo Yoo,Jaehee Lee,Seung Ick Cha,Chang Ho Kim,Sukki Cho,Sanghoon Jheon,Jae Yong Park

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PICOT antibody

Immunoprecipitation - Anti-PICOT antibody (AB226396)

Western blot - Anti-PICOT antibody (AB226396)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise