AB244349

Anti-PIEZO2 antibody

Name: Anti-PIEZO2 antibody
Brand: Abcam Limited
SKU: AB244349
Rating: 4 (2 reviews)

(2 리뷰들)

(1 출판물)

Rabbit Polyclonal PIEZO2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIEZO2 aa 2350 to C-terminus.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemical analysis of paraffin-embedded human cervix tissue labeling PIEZO2 with ab244349 used at 1/50 - 1/200 dilution. NOTE : The specified working dilutions of the primary antibodies are to be considered as a guideline only. Optimal dilutions must be determined by the user.

Uterine shows no positivity in squamous epithelial cells as expected.

The section was incubated with ab244349 for 30 mins at room temperature.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed using retrieval buffer pH 6.1 for 20 mins at 110°C in a pressure boiler.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling PIEZO2 with ab244349 used at 1/50 - 1/200 dilution. NOTE : The specified working dilutions of the primary antibodies are to be considered as a guideline only. Optimal dilutions must be determined by the user.

Moderate cytoplasmic positivity in urothelial cells.

The section was incubated with ab244349 for 30 mins at room temperature.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed using retrieval buffer pH 6.1 for 20 mins at 110°C in a pressure boiler.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PIEZO2 with ab244349 used at 1/50 - 1/200 dilution. NOTE : The specified working dilutions of the primary antibodies are to be considered as a guideline only. Optimal dilutions must be determined by the user.

Weak cytoplasmic positivity in glandular cells.

The section was incubated with ab244349 for 30 mins at room temperature.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed using retrieval buffer pH 6.1 for 20 mins at 110°C in a pressure boiler.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling PIEZO2 with ab244349 used at 1/50 - 1/200 dilution. NOTE : The specified working dilutions of the primary antibodies are to be considered as a guideline only. Optimal dilutions must be determined by the user.

Moderate cytoplasmic staining in neurons of human cerebral cortex.

The section was incubated with ab244349 for 30 mins at room temperature.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed using retrieval buffer pH 6.1 for 20 mins at 110°C in a pressure boiler.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Formalin-fixed, paraffin-embedded human colon tissue stained for PIEZO2 using ab244349 at 1/50 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PIEZO2 aa 2350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H5I5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PIEZO2 also known as FAM38B is a mechanosensitive ion channel protein. It has a high molecular mass of approximately 287 kDa. This protein exists in mechanosensitive tissues such as the skin bladder and lungs. PIEZO2 detects mechanical stimuli by opening its ion-conducting pore allowing the influx of cations into the cell which sends a signal.

Biological function summary

PIEZO2 plays an important role in the sensory pathways of touch and proprioception. It is not a part of a larger protein complex but operates as a homotrimer. The channel's sensitivity to mechanical forces enables cells to transduce physical touch into electrical signals facilitating an organism's ability to perceive its environment.

Pathways

Sensory transduction and mechanotransduction heavily involve PIEZO2. This protein interacts within pathways that transmit information about mechanical loads to the nervous system. Although not directly part of larger pathways' cascades its role is well observed in touch-related signaling paths where it occasionally interacts with proteins like TRPV1.

Mutations in PIEZO2 relate to Gordon Syndrome and Marden-Walker Syndrome. Dysfunction in PIEZO2 can lead to defects in touch and balance implicating disorders with sensory processing. There is also a notable connection to the protein TRPA1 which participates in similar sensory pathways and can correlate with sensory-related dysfunctions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain (PubMed : 37590348). Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways (PubMed : 37590348). Expressed in sensory neurons, is essential for diverse physiological processes, including respiratory control, systemic metabolism, urinary function, and proprioception (By similarity). Mediates airway stretch sensing, enabling efficient respiration at birth and maintaining normal breathing in adults (By similarity). It regulates brown and beige adipose tissue morphology and function, preventing systemic hypermetabolism (By similarity). In the lower urinary tract, acts as a sensor in both the bladder urothelium and innervating sensory neurons being required for bladder-stretch sensing and urethral micturition reflexes, ensuring proper urinary function (PubMed : 33057202). Additionally, PIEZO2 serves as the principal mechanotransducer in proprioceptors, facilitating proprioception and coordinated body movements (By similarity). In inner ear hair cells, PIEZO1/2 subunits may constitute part of the mechanotransducer (MET) non-selective cation channel complex where they may act as pore-forming ion-conducting component in the complex (By similarity). Required for Merkel-cell mechanotransduction (By similarity). Plays a major role in light-touch mechanosensation (By similarity).

See full target information PIEZO2

대체 명칭 보기

C18orf30, C18orf58, FAM38B, PIEZO2, Piezo-type mechanosensitive ion channel component 2, Protein FAM38B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Inflammation 46:1272-1289 PubMed36997763

2023

Dehydration Accelerates Cytogenesis and Cyst Growth in Pkd1 Mice by Regulating Macrophage M2 Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Jie Zhou,Dongjuan Zhang,Jiayi Lv,Meihan Chen,Chao Wang,Minghui Song,Fagui He,Shuwei Song,Changlin Mei

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PIEZO2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIEZO2 antibody (AB244349)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise