AB236405

Anti-PISD antibody [OTI4G5]

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(2 제품이 사용된 논문 )

Mouse Monoclonal PISD antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PISD aa 50 to C-terminus.

대체 명칭 보기

PISD

3 이미지

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PISD antibody [OTI4G5] (AB236405)

Paraffin-embedded human ovary adenocarcinoma tissue stained for PISD using ab236405 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PISD antibody [OTI4G5] (AB236405)

Paraffin-embedded human colon tissue stained for PISD using ab236405 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PISD antibody [OTI4G5] (AB236405)

Paraffin-embedded human lung tissue stained for PISD using ab236405 at 1/150 dilution in immunohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4G5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PISD aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UG56

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 °C for 3 minutes.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The phosphatidylserine decarboxylase (PISD) also called PSD is an enzyme that plays an important role in lipid metabolism within the cell. Its function centers on converting phosphatidylserine to phosphatidylethanolamine an important step in the synthesis of essential phospholipids. PISD with a molecular weight of approximately 45 kDa is expressed in multiple tissues with significant expression found in the mitochondria. This expression highlights its involvement in critical cellular processes related to membrane formation and maintenance.

Biological function summary

PISD acts on phosphatidylserine and contributes to the biosynthesis of phosphatidylethanolamine. The enzyme operates within a mitochondrial lipid biosynthetic pathway often forming part of a protein complex that ensures efficient catalytic activity. By facilitating phospholipid production PISD spans a range of cellular functions including structural support and signaling within the cell membrane. This lipid biosynthetic role is essential for maintaining cellular integrity and function.

Pathways

PISD operates within the glycerophospholipid metabolism and phospholipid biosynthesis pathways. These pathways support essential cellular operations such as membrane fluidity and cell signaling. Within these processes PISD collaborates with proteins like phosphatidylserine synthase 1 (PSS1) which synthesizes phosphatidylserine therefore providing the substrate for PISD activity. Alongside these enzymes the interplay in these pathways ensures the controlled production of phospholipids necessary for cellular homeostasis.

PISD dysfunction associates with metabolic and neurodegenerative conditions. Altered activity of PISD has been linked to mitochondrial disorders where inefficiencies in phospholipid metabolism disrupt mitochondrial function. This disruption can implicate proteins such as cardiolipin synthase which collaborates in the maintenance of mitochondrial integrity. Additionally PISD's role in lipid metabolism can influence conditions like non-alcoholic fatty liver disease where perturbations in lipid homeostasis lead to liver dysfunction. Understanding PISD's involvement offers insights into potential therapeutic targets for these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer) (PubMed : 30488656, PubMed : 30858161). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane (By similarity).

See full target information PISD

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular carcinogenesis 62:907-919 PubMed37157950

2023

PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Yunli Zhang,Shiyu Pang,Jingxian Min,Tao Wang,Dali Wu,Chun Lin,Zebin Xiao,Qi Xiang,Qing Li,Lili Ma

Nucleic acids research 49:7554-7570 PubMed34197606

2021

PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Peng,Ruifeng Shi,Jing Bian,Yuwei Li,Peipei Wang,Hailong Wang,Ji Liao,Wei-Guo Zhu,Xingzhi Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PISD antibody [OTI4G5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PISD antibody [OTI4G5] (AB236405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PISD antibody [OTI4G5] (AB236405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PISD antibody [OTI4G5] (AB236405)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise