AB244343

Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PITX2/RGS antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

ARP1, RGS, RIEG, RIEG1, PITX2, Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on rat pituitary (PMID : 15761027, PMID : 16859901, PMID : 11807026) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on mouse pituitary (PMID : 15761027, PMID : 16859901, PMID : 11807026) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Negative control.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control,no staining on mouse spleen (PMID : 11157981) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Negative control.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control no staining on rat liver (PMID : 11157981) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22417

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab244343 is the carrier-free version of ab221142.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PITX2 protein also known as paired-like homeodomain 2 plays a critical role in transcription regulation influencing gene expression. As a transcription factor PITX2 belongs to the bicoid-like homeobox transcription factor family and weighs approximately 32 kDa. It is expressed in several tissues including the eye brain heart and pituitary gland. This expression pattern suggests its involvement in various developmental processes and functions.

Biological function summary

PITX2 influences the development of several organs and is pivotal in left-right asymmetry during embryogenesis. By regulating gene expression it helps form structural components while ensuring proper tissue differentiation. PITX2 acts as a part of a transcriptional complex that facilitates its role in these developmental pathways. It interacts with other transcription factors and coactivators fine-tuning the transcriptional activities that guide embryonic organ development.

Pathways

PITX2 is an essential component of the Wnt signaling and heart development pathways. Within these pathways PITX2 regulates genes that participate in important cellular processes affecting cell fate and organ positioning. The protein interacts with other pathway members including β-catenin which mediates its transcriptional activities. These interactions allow it to influence the signaling cascades dictating correct cellular responses necessary for development.

PITX2 is associated with Axenfeld-Rieger syndrome and atrial fibrillation. Mutations or dysregulation of PITX2 can cause improper development of eye and craniofacial structures leading to Axenfeld-Rieger syndrome where it may act in concert with FOXC1 another transcription factor. Additionally abnormal PITX2 function links to atrial fibrillation where it affects the rhythmic patterns of the heart. This connection to cardiac regulation highlights its importance in maintaining normal physiological functions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May play a role in myoblast differentiation. When unphosphorylated, associates with an ELAVL1-containing complex, which stabilizes cyclin mRNA and ensuring cell proliferation. Phosphorylation by AKT2 impairs this association, leading to CCND1 mRNA destabilization and progression towards differentiation.. Isoform PTX2C. Involved in the establishment of left-right asymmetry in the developing embryo.

See full target information PITX2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Circulation. Heart failure 17:e011504 PubMed38910562

2024

Overexpression of in Cardiomyocytes Promotes Cardiac Reverse Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Xu,Hang Zhang,Yuan Chang,Xiumeng Hua,Xiao Chen,Yixuan Sheng,Dan Shan,Mengni Bao,Shengshou Hu,Jiangping Song

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (AB244343)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise