Anti-PIWIL1 antibody
5
(4 리뷰들)
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(46 제품이 사용된 논문 )
Rabbit Polyclonal PIWIL1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 46 publications.
대체 명칭 보기
HIWI, PIWIL1, Piwi-like protein 1
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody (AB12337)
ICC/IF image of ab12337 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12337 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
- WB
Project
Western blot - Anti-PIWIL1 antibody (AB12337)
ab12337 gave a positive signal in both mouse and rat testis lysates (positive controls). Bands were also observed in mouse and rat liver lysates (negative controls) that were higher in size to that observed in testis.
All lanes:
Western blot - Anti-PIWIL1 antibody (ab12337) at 1 µg/mL
Lane 1:
Testis (Mouse) Tissue Lysate at 10 µg
Lane 2:
Liver (Mouse) Tissue Lysate at 10 µg
Lane 3:
Testis (Rat) Tissue Lysate - normal tissue (<a href='/ko/products/unavailable/testis-rat-tissue-lysate-normal-tissue-ab29388'>ab29388</a>) at 10 µg
Lane 4:
Liver (Rat) Tissue Lysate at 10 µg
Secondary
All lanes:
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 98 kDa
Observed band size: 102 kDa,99 kDa
false
Reactivity 정보
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PIWIL1 contributes to maintaining genomic integrity through its role in RNA-mediated gene regulation. It forms part of the piRNA-induced silencing complex. This complex targets transposable elements and prevents their mobility an important function for genomic stability. PIWIL1's involvement in gametogenesis highlights its essential function in reproductive biology and cellular processes where piRNA pathways operate.
Pathways
The PIWIL1 protein plays significant roles within the piRNA pathway which is important for transposon silencing and germline integrity. PIWIL1 interacts with MILI and MIWI two proteins that also participate in the silencing of transposable elements. This pathway intersects with other gene regulation pathways influencing the overall expression landscape necessary for proper cellular function in reproductive tissues.
제품 프로토콜
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타겟 정보
제품이 사용된 논문 (46)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Scientific reports 14:15732 PubMed38977826
2024
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International journal of molecular sciences 24: PubMed37298298
2023
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Frontiers in oncology 11:783583 PubMed35083142
2022
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Molecular cell 81:4826-4842.e8 PubMed34626567
2021
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Frontiers in endocrinology 12:696121 PubMed34326815
2021
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BMC biology 19:86 PubMed33910563
2021
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Cell reports 34:108522 PubMed33406417
2021
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Cancer biology & therapy 21:1025-1032 PubMed33073675
2020
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Archives of gynecology and obstetrics 302:925-933 PubMed32613450
2020
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RNA biology 17:1613-1624 PubMed32372724
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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