AB151402

Anti-Plakophilin 2/PKP2 antibody [8H6]

Name: Anti-Plakophilin 2/PKP2 antibody [8H6]
Brand: Abcam Limited
SKU: AB151402
Rating: 4 (2 reviews)

(2 리뷰들)

(2 제품이 사용된 논문 )

Mouse Monoclonal Plakophilin 2/PKP2 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKP2 aa 1-350.

2 이미지

Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (AB151402)

Flow Cyt

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Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (AB151402)

Overlay histogram showing HeLa cells stained with ab151402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151402, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Western blot - Anti-Plakophilin 2/PKP2 antibody [8H6] (AB151402)

All lanes:

Western blot - Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402) at 1/100 dilution

All lanes:

HaCat Human keratinocyte cells

Predicted band size: 97 kDa

Observed band size: 92 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

8H6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human PKP2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q99959

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Plakophilin 2 also known as PKP2 is a protein integral to the function of cell junctions specifically within desmosomes. It is one of the plakophilins a subgroup of the armadillo family proteins. The protein has an approximate molecular weight of 97 kDa. PKP2 is extensively expressed in tissues where cells require strong mechanical adhesion such as cardiac and epidermal tissues. It plays an essential role by anchoring intermediate filaments contributing to structural integrity.

Biological function summary

PKP2 participates in forming and maintaining stable desmosomal connections in cells. It operates as part of the desmosome complex binding to cadherins and linking them to cytoskeletal filaments. This role is important for maintaining cell-cell adhesion during tissue stress particularly in tissues subject to mechanical stress such as the heart. Its interaction with cadherins and intermediate filaments highlights its role in managing cellular integrity and communication.

Pathways

PKP2 is fundamentally involved in cell signaling and junction-related pathways. It forms part of the desmosomal complex pathway which ensures robust cell adhesion and signaling integrity. PKP2 interacts with other proteins in the pathway such as desmoplakin and plakoglobin aiding mechanical stability and signal transduction necessary for tissue integrity and repair.

PKP2 mutations are closely associated with arrhythmogenic right ventricular cardiomyopathy (ARVC) a disease impacting cardiac function. The disorder links PKP2 to desmosomal dysfunction affecting heart muscle integrity and resulting in arrhythmias. Furthermore in the context of skin disorders PKP2 interactions with other desmosomal proteins like desmoglein might influence conditions involving epidermal fragility or blistering.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 25208567). Regulates focal adhesion turnover resulting in changes in focal adhesion size, cell adhesion and cell spreading, potentially via transcriptional modulation of beta-integrins (PubMed : 23884246). Required to maintain gingival epithelial barrier function (PubMed : 34368962). Important component of the desmosome that is also required for localization of desmosome component proteins such as DSC2, DSG2 and JUP to the desmosome cell-cell junction (PubMed : 22781308, PubMed : 25208567). Required for the formation of desmosome cell junctions in cardiomyocytes, thereby required for the correct formation of the heart, specifically trabeculation and formation of the atria walls (By similarity). Loss of desmosome cell junctions leads to mis-localization of DSP and DSG2 resulting in disruption of cell-cell adhesion and disordered intermediate filaments (By similarity). Modulates profibrotic gene expression in cardiomyocytes via regulation of DSP expression and subsequent activation of downstream TGFB1 and MAPK14/p38 MAPK signaling (By similarity). Required for cardiac sodium current propagation and electrical synchrony in cardiac myocytes, via ANK3 stabilization and modulation of SCN5A/Nav1.5 localization to cell-cell junctions (By similarity). Required for mitochondrial function, nuclear envelope integrity and positive regulation of SIRT3 transcription via maintaining DES localization at its nuclear envelope and cell tip anchoring points, and thereby preserving regulation of the transcriptional program (PubMed : 35959657). Maintenance of nuclear envelope integrity protects against DNA damage and transcriptional dysregulation of genes, especially those involved in the electron transport chain, thereby preserving mitochondrial function and protecting against superoxide radical anion generation (PubMed : 35959657). Binds single-stranded DNA (ssDNA) (PubMed : 20613778). May regulate the localization of GJA1 to gap junctions in intercalated disks of the heart (PubMed : 18662195). Involved in the inhibition of viral infection by influenza A viruses (IAV) (PubMed : 28169297). Acts as a host restriction factor for IAV viral propagation, potentially via disrupting the interaction of IAV polymerase complex proteins (PubMed : 28169297).

See full target information PKP2

대체 명칭 보기

Plakophilin-2, PKP2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology 27: PubMed40879390

2025

Comprehensive analysis of desmosomal protein remodelling identifies desmocollin-2 as potential biomarker for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Zhenliang Hu,Lingmin Wu,Deniz Akdis,Weiquan Ye,Ardan M Saguner,Mingming Su,Hongbin Dong,Zhongli Chen,Dan Hu,Shengshou Hu,Firat Duru,Liang Chen

Experimental and therapeutic medicine 15:2255-2262 PubMed29456632

2018

Arrhythmogenic cardiomyopathy: Identification of desmosomal gene variations and desmosomal protein expression in variation carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Shenghua Liu,Hongliang Zhang,Shengshou Hu,Yingjie Wei

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Plakophilin 2/PKP2 antibody [8H6]

Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (AB151402)

Western blot - Anti-Plakophilin 2/PKP2 antibody [8H6] (AB151402)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise