Anti-Plasma Kallikrein 1B antibody [RM2034]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Plasma Kallikrein 1B with ab315396 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma of human kidney. The section was incubated with ab315396 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Plasma Kallikrein 1B with ab315396 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on human spleen. The section was incubated with ab315396 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Plasma Kallikrein 1B with ab315396 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma of human lung. The section was incubated with ab315396 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Plasma Kallikrein 1B with ab315396 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma of mouse kidney. The section was incubated with ab315396 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Plasma Kallikrein 1B with ab315396 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma of rat kidney. The section was incubated with ab315396 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 30559188), liver.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30559188, 29489063).

The identity of the lower MW band at approximately 36 kDa is light chain of Plasma Kallikrein 1B.

The bands above 80 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (ab315396) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human plasma at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, brain (PMID : 30559188), liver.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30559188, 29489063).

The identity of the lower MW band at approximately 36 kDa is light chain of Plasma Kallikrein 1B.

The bands above 80 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (ab315396) at 1/1000 dilution

Lane 1:

Rat plasma at 20 µg

Lane 2:

Rat serum at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, brain (PMID : 30559188), liver.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30559188, 29489063).

The identity of the lower MW band at approximately 36 kDa is light chain of Plasma Kallikrein 1B.

The bands above 80 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (ab315396) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 26s

• Dot

Supplier Data

Dot Blot - Anti-Plasma Kallikrein 1B antibody [RM2034] (AB315396)

Dot blot analysis of Plasma Kallikrein 1B using ab315396 at 1 : 1000 (0.497 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse Coagulation factor XI.