Anti-PLBD2 antibody [EPR21204]

• WB

Lab

Western blot - Anti-PLBD2 antibody [EPR21204] (AB225580)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA solution before being incubated with ab225580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-PLBD2 antibody [EPR21204] (ab225580) at 1 µg/mL

Lane 1:

Human heart tissue lysate at 10 µg

Lane 2:

Human cerebellum tissue lysate at 10 µg

Lane 3:

Mouse cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 65 kDa

Observed band size: 45 kDa

true

Exposure time: 1min

• I-ELISA

Lab

Indirect ELISA - Anti-PLBD2 antibody [EPR21204] (AB225580)

Different concentrations of immobilised PLBD2 protein was recognised in an indirect ELISA by ab225580. Bound ab225580 (1 μg x mL-1) was detected by peroxidase conjugated goat anti-rabbit IgG (0.16 μg x mL-1). OD (background signal subtracted) shown on the y-axis.