AB115514

Anti-Pleckstrin antibody

Name: Anti-Pleckstrin antibody
Brand: Abcam Limited
SKU: AB115514
Rating: 3 (1 reviews)

(1 리뷰)

(2 제품이 사용된 논문 )

Goat Polyclonal Pleckstrin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PLEK aa 300 to C-terminus.

대체 명칭 보기

P47, PLEK, Pleckstrin, Platelet 47 kDa protein, p47

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human vessel (platelets) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-Pleckstrin antibody (AB115514)

Primary incubation : 1 hour

All lanes:

Western blot - Anti-Pleckstrin antibody (ab115514) at 0.05 µg/mL

All lanes:

Human PBMC lysate (in RIPA buffer) at 35 µg

Predicted band size: 40 kDa

true

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PLEK aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08567

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05-1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pleckstrin also known as PLEK is a phosphoprotein with a mass of approximately 40 kDa. It is predominantly expressed in platelets and leukocytes. Pleckstrin is the major protein kinase C substrate in platelets and acts as an important mediator in signal transduction processes. Its structure includes pleckstrin homology domains which play key roles in interacting with other cellular proteins and in lipid binding.

Biological function summary

Pleckstrin participates in cellular processes like cytoskeletal rearrangement and cell motility. It functions as part of protein signaling complexes that regulate actin cytoskeleton dynamics. Through its pleckstrin homology domains pleckstrin binds to phosphatidylinositol lipids which is critical for recruitment to membrane sites required for cellular activation and response.

Pathways

Pleckstrin is intricately involved in the PI3K/AKT signaling pathway and the platelet activation pathway. Within these pathways pleckstrin interacts with proteins like G-protein coupled receptors and PI3K facilitating cellular responses to external stimuli. These processes ensure platelets function correctly during hemostasis and inflammatory responses.

Pleckstrin's role is critical in diseases like thrombocytopenia and psoriasis. Aberrations in pleckstrin expression or function can lead to improper platelet function contributing to abnormal bleeding or clotting scenarios in thrombocytopenia. It is also connected with proteins like PIP3 and AKT which are involved in pathways that can become dysregulated in chronic inflammatory disorders such as psoriasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Major protein kinase C substrate of platelets.

See full target information PLEK

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine (Cambridge, Mass.) 28:76 PubMed35768768

2022

Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Yin,Yaofa Lin,Peilin Wang,Jun Zhou,Haodong Lin

Human molecular genetics 29:1520-1536 PubMed32337552

2020

A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Lezirovitz,Gleiciele A Vieira-Silva,Ana C Batissoco,Débora Levy,Joao P Kitajima,Alix Trouillet,Ellen Ouyang,Navid Zebarjadi,Juliana Sampaio-Silva,Vinicius Pedroso-Campos,Larissa R Nascimento,Cindy Y Sonoda,Vinícius M Borges,Laura G Vasconcelos,Roberto M O Beck,Signe S Grasel,Daniel J Jagger,Nicolas Grillet,Ricardo F Bento,Regina C Mingroni-Netto,Jeanne Oiticica

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pleckstrin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

Western blot - Anti-Pleckstrin antibody (AB115514)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise