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AB325938

Anti-PLK4 antibody [EPR30475-69]

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Rabbit Recombinant Monoclonal PLK4 antibody. Suitable for ICC/IF and reacts with Mouse samples.
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Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody [EPR30475-69] (AB325938)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody [EPR30475-69] (AB325938)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) NIH/3T3 treated with CFI-400945(500nM) for 18hours cells labelling PLK4 with ab325938 at 1/50 (10.48 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing centrosome staining in NIH/3T3 cells treated with CFI-400945 ( 500nM ) for 18 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody [EPR30475-69] (AB325938)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody [EPR30475-69] (AB325938)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) NIH/3T3 treated with CFI-400945 ( 500nM ) for18 hours cells labelling PLK4 with ab325938 at 1/50 (10.48 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing centrosome staining in NIH/3T3 cells treated with CFI-400945 ( 500nM ) for 18 hours (shown in green), which is co-stained with a centrosome marker. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab307294 Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30475-69

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB89314

Anti-STIL/SIL antibody

Immunoprecipitation - Anti-STIL/SIL antibody (AB89314)

  • 0
  • 0
Primary Antibodies

AB220784

Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

  • 4
  • 2
Primary Antibodies

AB230818

Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235]

RabMAb|Recombinant|Lab Essentials|Trial Size

Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (AB230818)

  • 0
  • 0
Primary Antibodies

AB16663

Anti-Cyclin D1 antibody [SP4]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [SP4] (AB16663)

  • 4
  • 23
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (By similarity). Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites (By similarity).
See full target information Plk4

대체 명칭 보기

Sak, Stk18, Serine/threonine-protein kinase PLK4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak, PLK-4
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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