AB236976

Anti-PLS1 antibody

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Polyclonal PLS1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLS1 aa 1-150.

대체 명칭 보기

Plastin-1, Intestine-specific plastin, I-plastin, PLS1

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-PLS1 antibody (AB236976)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PLS1 antibody (AB236976)

A549 (human lung carcinoma cell line) cells stained for PLS1 (green) using ab236976 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained wit DAPI.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

Paraffin-embedded human small intestine tissue stained for PLS1 using ab236976 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

Paraffin-embedded human kidney tissue stained for PLS1 using ab236976 at 1/400 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PLS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q14651

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PLS1 also known as Plastin-1 or fimbrin acts as a pivotal actin-bundling protein influencing the dynamic restructuring of the actin cytoskeleton. This protein has a known molecular mass of approximately 70 kDa. Within human tissues PLS1 is mainly expressed in the small intestine and inner ear supporting its roles in processes like cellular movement and structural integrity. Studies identify it within the microvillar structures attributing to its function in maintaining microvillar core actin filaments.

Biological function summary

PLS1 influences cellular stability by interacting with actin filaments and linking them into organized bundles. This protein engages within microvilli by enhancing the rigidity and static structure necessary for absorption and secretion processes. PLS1 does not form larger protein complexes but operates intimately with actin. Its bundling ability is essential in maintaining the structural organization of actin-rich cellular extensions like filopodia and microvilli.

Pathways

PLS1 plays an integral role in the regulation of actin cytoskeleton pathways. Its action affects pathways such as the cytoskeletal regulation by Rho GTPases which are vital for cellular shape and movement. Within these pathways PLS1 interacts with actin-related proteins such as cofilin supporting the reorganization and stabilization of actin filament networks essential in cellular morphological changes and motility.

The dysregulation of PLS1 can impact conditions like hereditary hearing loss and intestinal disorders. It is associated with syndromic forms of hearing loss due to its expression in the inner ear where proper actin filament organization impacts auditory function. Additionally PLS1's role in maintaining microvillar structure ties to intestinal pathologies. There is interaction with proteins like myosin VIIA in these contexts where the collaborative function with PLS1 influences microvillus stability impacting hearing-related cellular organization and intestinal absorption efficiency.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Actin-bundling protein. In the inner ear, it is required for stereocilia formation. Mediates liquid packing of actin filaments that is necessary for stereocilia to grow to their proper dimensions.

See full target information PLS1

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncogene 41:997-1010 PubMed34974522

2022

Novel function of THEMIS2 in the enhancement of cancer stemness and chemoresistance by releasing PTP1B from MET.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chieh Huang,Jia-Hau Yen,Yu-Wen Sung,Shiao-Lin Tung,Po-Ming Chen,Pei-Yi Chu,Ya-Chi Shih,Hsiang-Cheng Chi,Yi-Ching Huang,Shih-Jei Huang,Lu-Hai Wang

Aging 12:9549-9584 PubMed32454462

2020

MiR-1908/EXO1 and MiR-203a/FOS, regulated by scd1, are associated with fracture risk and bone health in postmenopausal diabetic women.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Sheng Chen,Xue-Ran Kang,Zi-Hui Zhou,Jiang Yang,Qi Xin,Chen-Ting Ying,Yun-Peng Zhang,Jie Tao

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PLS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PLS1 antibody (AB236976)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise