Anti-PLVAP/PV-1 antibody [EPR29045-583]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] (AB323706)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling PLVAP/PV-1 with ab323706 at 1/2000 (0.239 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive on endothelial cells in human clear cell carcinoma. The section was incubated with ab323706 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] (AB323706)

Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling PLVAP/PV-1 with ab323706 at 1/2000 (0.239 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive on endothelial cells in human cervical squamous carcinoma. The section was incubated with ab323706 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] (AB323706)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLVAP/PV-1 with ab323706 at 1/2000 (0.239 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive on endothelial cells in human colon (PMID : 31419514). The section was incubated with ab323706 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] (AB323706)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum, testis(PMID : 11401446)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30854484).

The identity of the bands lower than 70 kDa in lanes 1-2 are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] (ab323706) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate at 20 µg

Lane 2:

Human lymph node tissue lysate at 20 µg

Lane 3:

Human lung cancer tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Lane 5:

Human cerebellum tissue lysate at 20 µg

Lane 6:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 70 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] (AB323706)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Raji, MCF7.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] (ab323706) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 70 kDa,36 kDa

false

Exposure time: 180s