AB270406

Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PMP22 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PMP22 with ab270399 at 1 : 16000 dilution (0.03 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : negative staining in human kidney (PMID : 7649472).

The section was incubated with ab270399 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270399).

Flow Cytometry - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Flow Cyt

Lab

Flow Cytometry - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T transfected with myc-his tagged PMP22 overexpression construct cells labelling PMP22 with ab270399 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with isotype control or ab270399. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270399).

Immunocytochemistry/ Immunofluorescence - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T+OE-637(+/-) cells labelling PMP22 with ab270399 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with a myc-tagged PMP22 expression construct is observed. 2233S Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270399).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Immunohistochemical analysis of paraffin-embedded Human nerve tissue labeling PMP22 with ab270399 at 1/16000 dilution (0.03 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in Schwann cells of human nerve (PMID : 7691737) is observed. The section was incubated with ab270399 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270399).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23113-31

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab270406 is the carrier-free version of ab270399.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 'PMP22 protein' also known as Peripheral Myelin Protein 22 is an essential component of the myelin sheath in the peripheral nervous system. It has a molecular weight of approximately 22 kDa. The protein is highly expressed in Schwann cells which are responsible for forming myelin around peripheral nerves. PMP22 contains four transmembrane domains playing a role in its structural function in the membrane.

Biological function summary

The 'PMP22' serves an important role in maintaining the integrity and functionality of the myelin sheath contributing to efficient nerve conduction. It is not typically part of a complex but interacts with other myelin proteins to support myelin compaction and stability. The protein's presence affects the cell cycle and myelination process important for the normal functioning of the peripheral nervous system.

Pathways

'PMP22 protein' plays roles in the Schwann cell differentiation and myelination pathways. It maintains correct peripheral nerve structure and function with connections to proteins such as MPZ (Myelin Protein Zero) which are also integral for proper nerve insulation. There are specific pathways where PMP22 downregulation or overexpression impacts the normal myelination cycle significant for peripheral nerve health.

'PMP 22' mutations or overexpression relate to Charcot-Marie-Tooth disease type 1A and Hereditary Neuropathy with Liability to Pressure Palsies (HNPP). These conditions arise from gene dosage changes affecting nerve function where altered PMP22 protein levels lead to defective myelin formation. Interaction between PMP22 and proteins like connexin 32 shows further ties to demyelinating neuropathies emphasizing the gene-protein network involved in these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Might be involved in growth regulation, and in myelinization in the peripheral nervous system.

See full target information PMP22

대체 명칭 보기

GAS3, PMP22, Peripheral myelin protein 22, PMP-22, Growth arrest-specific protein 3, GAS-3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Flow Cytometry - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Immunocytochemistry/ Immunofluorescence - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23113-31] - BSA and Azide free (AB270406)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise