AB140171

Anti-PMPCA/INPP5E antibody

Name: Anti-PMPCA/INPP5E antibody
Brand: Abcam Limited
SKU: AB140171
Rating: 5 (1 reviews)

(1 리뷰)

(2 제품이 사용된 논문 )

Rabbit Polyclonal PMPCA/INPP5E antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PMPCA aa 150-400.

대체 명칭 보기

INPP5E, KIAA0123, MPPA, PMPCA, Mitochondrial-processing peptidase subunit alpha, Alpha-MPP, Inactive zinc metalloprotease alpha, P-55

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMPCA/INPP5E antibody (AB140171)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMPCA/INPP5E antibody (AB140171)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal colon tissue labelling PMPCA/INPP5E with ab140171 at 1/100 dilution.

AbReview60151****

Western blot - Anti-PMPCA/INPP5E antibody (AB140171)

Protein standard (bands from the top to the bottom : 250, 150, 100, 75 (yellow), 50, 37, 25 (yellow), 20, 15 kDa.

Blocked for 1 hour at 25°C. Incubated with the primary antibody for 16 hours at 4°C.

All lanes:

Western blot - Anti-PMPCA/INPP5E antibody (ab140171) at 1/1000 dilution

Lane 1:

Mouse whole brain homogenate at 20 µg

Lane 2:

Mice brain non-synaptosomal mitochondria at 20 µg

Lane 3:

Mitochondria from ST-Hdh-Q7/Q7 striatal cells at 20 µg

Lane 4:

Mitochondria from ST-Hdh-Q111/Q111 striatal cells at 20 µg

Lane 5:

HEK293 whole cell homogenate at 20 µg

Lane 6:

Mitochondria from HEK293 cells at 20 µg

Secondary

All lanes:

IRDye 800CY-conjugated monoclonal goat anti-rabbit IgG at 1/15000 dilution

Predicted band size: 58 kDa

Observed band size: 40 kDa,50 kDa

false

Exposure time: 5min

This image is courtesy of an Abreview submitted by Svitlana Yablonska.

Unknown

Western blot - Anti-PMPCA/INPP5E antibody (AB140171)

All lanes:

Western blot - Anti-PMPCA/INPP5E antibody (ab140171) at 1/1000 dilution

Lane 1:

MCF7 cell lysate

Lane 2:

A549 cell lysate

Predicted band size: 58 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PMPCA aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q10713

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PMPCA (peptidase (mitochondrial processing) beta) and INPP5E (inositol polyphosphate-5-phosphatase E) are proteins involved in distinct cellular functions. PMPCA is a subunit of a mitochondrial processing peptidase that weighs approximately 56 kDa. It is primarily expressed in the mitochondria where it processes precursor proteins by cleaving specific N-terminal sequences to facilitate proper protein maturation. INPP5E is a lipid phosphatase localized to cilia and cytoplasm with a mass of about 72 kDa playing a role in phosphoinositide signaling pathways.

Biological function summary

The proteins perform unique functions essential to cell homeostasis and function. PMPCA plays a role in mitochondrial protein maintenance and is necessary for mitochondrial integrity. INPP5E engages in phosphoinositide metabolism and is part of a multi-protein complex within primary cilia. INPP5E regulates phosphoinositide levels important for ciliary function and signaling impacting various downstream biological processes.

Pathways

PMPCA is integrated into the mitochondrial import pathway important for functional validation of mitochondrial metabolic and respiratory pathways. It interacts with proteins like HSP60 and HSP10. INPP5E influences the phosphoinositide-dependent signaling pathway vital for maintaining the stability and function of primary cilia. This pathway associates with proteins such as tubby-like protein 3 (TULP3) orchestrating ciliary membrane signaling events.

PMPCA relates to neurodegenerative conditions such as spinocerebellar ataxia due to its role in ensuring mitochondrial protein processing. Connections exist with proteins like mitochondrial DNA-directed RNA polymerase contributing to the disorder. INPP5E is linked to ciliopathies like Joubert syndrome because of abnormalities in ciliary phosphoinositide levels. The involvement of INPP5E in these disorders often relates to its interaction with the ARL13B protein which influences ciliary biology and pathogenesis.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.

See full target information PMPCA

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cellular and molecular life sciences : CMLS 80:258 PubMed37594630

2023

Protease-independent control of parthanatos by HtrA2/Omi.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Weiß,Michelle Heib,Thiemo Korn,Justus Hoyer,Johaiber Fuchslocher Chico,Susann Voigt,Tomas Koudelka,Andreas Tholey,Dieter Adam

Molecular therapy : the journal of the American Society of Gene Therapy 29:1001-1015 PubMed33221434

2020

CRISPR/Cas9-Mediated Gene Correction in Newborn Rabbits with Hereditary Tyrosinemia Type I.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Shixue Gou,Jiaowei Wang,Quanjun Zhang,Xingyun Huang,Jingke Xie,Li Li,Qin Jin,Zhen Ouyang,Fangbing Chen,Weikai Ge,Hui Shi,Yanhui Liang,Zhenpeng Zhuang,Xiaozhu Zhao,Meng Lian,Yinghua Ye,Longquan Quan,Han Wu,Liangxue Lai,Kepin Wang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PMPCA/INPP5E antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMPCA/INPP5E antibody (AB140171)

Western blot - Anti-PMPCA/INPP5E antibody (AB140171)

Western blot - Anti-PMPCA/INPP5E antibody (AB140171)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Sample Prep & Detection Kits

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Reconstitution

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise