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AB326043

Anti-PMS2 antibody [EPR30476-81]

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Rabbit Recombinant Monoclonal PMS2 antibody. Suitable for WB and reacts with Mouse samples.
2 이미지
Western blot - Anti-PMS2 antibody [EPR30476-81] (AB326043)
  • WB

Lab

Western blot - Anti-PMS2 antibody [EPR30476-81] (AB326043)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands between 20 kDa and 25 kDa are unknown.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-PMS2 antibody [EPR30476-81] (ab326043) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Neuro-2a transfected with siRNA specifically targeting PMS2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 96 kDa,36 kDa

false

Exposure time: 15s

Western blot - Anti-PMS2 antibody [EPR30476-81] (AB326043)
  • WB

Lab

Western blot - Anti-PMS2 antibody [EPR30476-81] (AB326043)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands between 20 kDa and 25 kDa in lane 1 are unknown.

The bands beneath the target band (96 kDa) may represent degraded target fragments.

To minimize protein degradation, cells/tissues in lanes 1-2 were lysed immediately after harvest and applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-PMS2 antibody [EPR30476-81] (ab326043) at 1/1000 dilution

Lane 1:

Mouse testis tissue fresh lysate at 20 µg

Lane 2:

F9 (mouse embryonal carcinoma epithelial cell) fresh whole cell lysate at 20 µg

Lane 3:

F9 (mouse embryonal carcinoma epithelial cell) frozen whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 96 kDa

false

Exposure time: 59s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30476-81

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB92312

Anti-MLH1 antibody [EPR3894]

RabMAb|Recombinant|KO Validated|20ul selling size

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Primary Antibodies

AB92471

Anti-MSH6 antibody [EPR3945]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (AB92471)

  • 5
  • 5
Primary Antibodies

AB227941

Anti-MSH2 antibody [EPR21017-123]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MSH2 antibody [EPR21017-123] (AB227941)

  • 0
  • 0
Primary Antibodies

AB230818

Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235]

RabMAb|Recombinant|Lab Essentials|Trial Size|Trial Size

Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (AB230818)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair (By similarity).
See full target information Pms2

대체 명칭 보기

Mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 protein homolog 2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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