AB70248

Anti-PNUTS antibody

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Rabbit Polyclonal PNUTS antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

2 이미지

Unknown

Immunoprecipitation - Anti-PNUTS antibody (AB70248)

Immunoprecipitation/ Western Blot of PNUTS

Lane 1 : Another antibody which recognizes a different epitope of PNUTS.

Lane 2 : Another antibody which recognizes a different epitope of PNUTS.

Lane 3 : Another antibody which recognizes a different epitope of PNUTS.

Lane 4 : ab70248 at 1μg/100μg nuclear extract.

Detection : Chemiluminescence with exposure times of 3 seconds.

Lane 1:

Immunoprecipitation - Anti-PNUTS antibody (ab70248)

Lane 1:

Immunoprecipitation - Anti-PNUTS antibody (<a href='/ko/products/primary-antibodies/pnuts-antibody-ab70247'>ab70247</a>)

Predicted band size: 99 kDa

false

Unknown

Western blot - Anti-PNUTS antibody (AB70248)

Lanes 1 - 2:

Western blot - Anti-PNUTS antibody (ab70248) at 1 µg/mL

Lanes 3 - 4:

Western blot - Anti-PNUTS antibody (ab70248) at 0.2 µg/mL

Lanes 1 and 3:

Nuclear extracts from Hela cells at 15 µg

Lanes 2 and 4:

Nuclear extracts from Hela cells at 45 µg

Predicted band size: 99 kDa

Observed band size: 115 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Medaka fish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PNUTS protein also known as 'little PNUTS' acts as a regulatory subunit in the cell. It plays a role in controlling the activity of protein phosphatase 1 (PP1) essential for various cellular processes. The PNUTS protein has a molecular mass of approximately 106 kDa. It is expressed in multiple tissues with significant presence in the brain and heart suggesting its importance in these areas.

Biological function summary

The PNUTS protein influences many cellular events as it is part of a larger complex. It controls the dephosphorylation activity of PP1 impacting processes like cell cycle regulation and chromatin structure modification. This protein contributes to the dynamic regulation of transcription DNA repair and cell survival. Its ability to interact with other proteins positions it as a versatile component in cell biology.

Pathways

The PNUTS protein interacts in several notable pathways that affect cell function. It is involved in the DNA damage response pathway where it regulates DNA repair through its interaction with components like PP1. It also plays a role in the Rb signaling pathway related to cell cycle progression linking its function to cell growth and division. Through these pathways PNUTS connects with other proteins emphasizing its versatility.

Alterations in the PNUTS protein correlate with various conditions. Its dysfunction is associated with cancer impacting cell proliferation through its role in the Rb pathway. The connection between PNUTS and neurodegenerative disorders also emerges due to its high expression in the brain. In diseases like Alzheimer's PNUTS interactions with proteins like tau influence disease progression making it a target of interest in therapeutic research.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Substrate-recognition component of the PNUTS-PP1 protein phosphatase complex, a protein phosphatase 1 (PP1) complex that promotes RNA polymerase II transcription pause-release, allowing transcription elongation (PubMed : 39603239, PubMed : 39603240). Promoter-proximal pausing by RNA polymerase II is a transcription halt following transcription initiation but prior to elongation, which acts as a checkpoint to control that transcripts are favorably configured for transcriptional elongation (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex mediates the release of RNA polymerase II from promoter-proximal region of genes by catalyzing dephosphorylation of proteins involved in transcription, such as AFF4, CDK9, MEPCE, INTS12, NCBP1, POLR2M/GDOWN1 and SUPT6H (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex also regulates RNA polymerase II transcription termination by mediating dephosphorylation of SUPT5H in termination zones downstream of poly(A) sites, thereby promoting deceleration of RNA polymerase II transcription (PubMed : 31677974). PNUTS-PP1 complex is also involved in the response to replication stress by mediating dephosphorylation of POLR2A at 'Ser-5' of the CTD, promoting RNA polymerase II degradation (PubMed : 33264625). The PNUTS-PP1 complex also plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (By similarity). PNUTS-PP1 complex mediates dephosphorylation of MYC, promoting MYC stability by preventing MYC ubiquitination by the SCF(FBXW7) complex (PubMed : 30158517). In addition to acts as a substrate-recognition component, PPP1R10/PNUTS also acts as a nuclear targeting subunit for the PNUTS-PP1 complex (PubMed : 9450550). In some context, PPP1R10/PNUTS also acts as an inhibitor of protein phosphatase 1 (PP1) activity by preventing access to substrates, such as RB (PubMed : 18360108).

See full target information PPP1R10

대체 명칭 보기

CAT53, FB19, PNUTS, PPP1R10, Serine/threonine-protein phosphatase 1 regulatory subunit 10, MHC class I region proline-rich protein CAT53, PP1-binding protein of 114 kDa, Phosphatase 1 nuclear targeting subunit, p99

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular pharmacology 95:120-126 PubMed30397001

2018

Cell Cycle and Apoptosis Regulator 1, CCAR1, Regulates Enhancer-Dependent Nuclear Receptor CAR Transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Kanno,Shuai Zhao,Naoya Yamashita,Nao Saito,Aoi Ujiie,Rie Iijima,Nami Kikawa,Kiyomitsu Nemoto,Yoshio Inouye

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PNUTS antibody

Immunoprecipitation - Anti-PNUTS antibody (AB70248)

Western blot - Anti-PNUTS antibody (AB70248)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise