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AB110876

Anti-POLE antibody

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Rabbit Polyclonal POLE antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLE.

대체 명칭 보기

POLE1, POLE, DNA polymerase epsilon catalytic subunit A, 3'-5' exodeoxyribonuclease, DNA polymerase II subunit A

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (AB110876)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (AB110876)

ab110876, at a 1/50 dilution, staining POLE in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunizing peptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human POLE. The exact immunogen used to generate this antibody is proprietary information.

Q07864

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대체품

Primary Antibodies

AB226848

Anti-POLE antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody - N-terminal (AB226848)

  • 0
  • 0

보완 제품

Primary Antibodies

AB186407

Anti-POLD1 antibody [EPR15118]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (AB186407)

  • 0
  • 0
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB31777

Anti-DNA polymerase alpha/POLA antibody

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

  • 4
  • 3
Primary Antibodies

AB108928

Anti-FANCD2 antibody [EPR2302]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2302] (AB108928)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The POLE protein also known as DNA polymerase epsilon catalytic subunit A functions as an important component in DNA replication and repair mechanisms. POLE possesses a molecular mass of approximately 261 kDa and demonstrates expression primarily in proliferating cells such as those in the bone marrow lymphoid tissues and the gastrointestinal tract. It operates within the eukaryotic DNA polymerase family distinct from other polymerases due to its high fidelity in DNA synthesis which minimizes errors during replication.
Biological function summary

The POLE protein plays an essential role in the high-fidelity synthesis of leading-strand DNA during replication. POLE is part of a larger complex known as the DNA polymerase epsilon holoenzyme collaborating with accessory proteins that enhance its ability to synthesize DNA accurately. Moreover this protein contributes significantly to the proofreading function as it has intrinsic 3' to 5' exonuclease activity allowing the removal of incorrectly paired nucleotides therefore preventing mutations.

Pathways

The POLE protein is fundamentally involved in the DNA replication and repair pathways. These pathways ensure genomic integrity and stability important for normal cell division and preventing genomic instability associated with cancer. POLE interacts with other proteins such as PCNA and RFC (replication factor C) within these pathways facilitating efficient replication fork progression and repair processes. POLE's activity ensures coordination with leading strand synthesis complementing the functions of other polymerases involved in lagging strand DNA synthesis.

Mutations in the POLE gene have been linked to colorectal cancer and endometrial cancer. These conditions are often characterized by a hypermutated phenotype arising from impaired DNA proofreading ability due to ineffective exonuclease activity. In this context POLE mutations can assume significance in sporadic cancer cases as well as in hereditary cancer syndromes where it interacts with mismatch repair proteins highlighting its role in maintaining genomic fidelity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalytic component of the DNA polymerase epsilon complex (PubMed : 10801849). Participates in chromosomal DNA replication (By similarity). Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity). Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (By similarity). Involved in DNA synthesis during DNA repair (PubMed : 20227374, PubMed : 27573199). Along with DNA polymerase POLD1 and DNA polymerase POLK, has a role in excision repair (NER) synthesis following UV irradiation (PubMed : 20227374).
See full target information POLE
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

European journal of human genetics : EJHG 23:1080-4 PubMed25370038

2014

Germline variants in POLE are associated with early onset mismatch repair deficient colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fadwa A Elsayed,C Marleen Kets,Dina Ruano,Brendy van den Akker,Arjen R Mensenkamp,Melanie Schrumpf,Maartje Nielsen,Juul T Wijnen,Carli M Tops,Marjolijn J Ligtenberg,Hans F A Vasen,Frederik J Hes,Hans Morreau,Tom van Wezel
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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