AB250160

Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal POLR2E/rpb5 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E

4 이미지

Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

This data was developed using ab180151, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E/rpb5 with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

This data was developed using ab180151, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E/rpb5 with ab180151 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

This data was developed using ab180151, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (<a href='/ko/products/primary-antibodies/polr2e-rpb5-antibody-epr11750-ab180151'>ab180151</a>) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

This data was developed using ab180151, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (<a href='/ko/products/primary-antibodies/polr2e-rpb5-antibody-epr11750-ab180151'>ab180151</a>) at 1/20000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11750

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab250160 is the carrier-free version of ab180151.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

POLR2E also known as RPB5 is a subunit of RNA polymerase II which plays a central role in the transcription of DNA into RNA. It weighs approximately 25 kDa. POLR2E is expressed in ubiquitously in human tissues indicating its essential role in basic cellular functions. As a component of the core RNA polymerase II complex it interacts with other polymerase subunits to form the enzyme that synthesizes messenger RNA (mRNA) precursors and many functional non-coding RNAs.

Biological function summary

The function of POLR2E contributes to transcription initiation and elongation processes. It is part of the larger multiprotein complex of RNA polymerase II which consists of twelve different subunits. This complex is responsible for transcribing mRNA from DNA templates in eukaryotic cells. POLR2E enables the assembly of the transcriptional machinery significantly affecting the regulation of gene expression.

Pathways

POLR2E operates in the transcription and mRNA processing pathways. It plays an important role in the synthesis of RNA transcripts that undergo splicing polyadenylation and capping before they become mature mRNAs for protein synthesis. POLR2E interacts with other factors in the transcription complex such as TFIIF and TFIIE which modulate transcriptional efficiency and fidelity.

POLR2E has been linked to transcription-related pathologies such as certain cancers and neurological disorders. Alterations in the expression or function of POLR2E can affect the proper transcription process leading to disrupted cellular functions. It also connects to proteins like BRCA1 in breast cancer where changes in gene expression play a role in tumor development and progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPABC1 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template.

See full target information POLR2E

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (AB250160)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise