AB235963

Anti-Polycystin 1/PC1 antibody

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Rabbit Polyclonal Polycystin 1/PC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKD1 aa 600 to C-terminus.

대체 명칭 보기

Polycystin-1, PC1, Autosomal dominant polycystic kidney disease 1 protein, PKD1

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 1/PC1 antibody (AB235963)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 1/PC1 antibody (AB235963)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Polycystin 1/PC1 (green) using ab235963 at 1/200 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody (AB235963)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody (AB235963)

Paraffin-embedded human colon cancer tissue stained for Polycystin1/PC1 using ab235963 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PKD1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P98161

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Polycystin 1 also called PC1 is a large transmembrane protein with a molecular mass of approximately 460 kDa. It primarily expresses in the epithelial cells of renal tubules and also in other tissues like liver and pancreas. PC1 has a role as a mechanosensor and regulates cellular adhesion along with extracellular matrix interactions. It works by forming a complex with another protein known as Polycystin-2 (PC2) and this interaction is important for its function.

Biological function summary

PC1 is involved in processes essential for normal kidney function and development. It is part of a complex that mediates calcium ion entry impacting signaling pathways that regulate cell proliferation differentiation and fluid secretion. This protein's interaction with PC2 through the PC1/PC2 complex is fundamental for its role in these cellular processes. By affecting calcium channels PC1 influences various cell signaling events critical for maintaining cellular homeostasis.

Pathways

PC1 participates in calcium signaling pathways and mechanotransduction. Its role in these pathways links PC1 with the regulation of intracellular calcium levels which is influential in cellular responses to mechanical stimuli. Polycystin-2 is closely associated with PC1 in these pathways as they form a functional polycystin complex that is necessary for these signaling mechanisms.

PC1 is significantly associated with autosomal dominant polycystic kidney disease (ADPKD). Mutations in the PC1 gene (PKD1) lead to cyst formation in kidneys and liver indicating its significant role in pathogenesis. Furthermore interactions between PC1 and PC2 are important as abnormalities in either protein can result in ADPKD. Understanding these interactions and the pathways in which PC1 is involved provides insights into potential therapeutic targets for treating related disorders.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B (PubMed : 27214281). Both PKD1 and PKD2 are required for channel activity (PubMed : 27214281). Involved in renal tubulogenesis (PubMed : 12482949). Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (By similarity). Acts as a regulator of cilium length, together with PKD2 (By similarity). The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling (By similarity). The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling (By similarity). May be an ion-channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Likely to be involved with polycystin-1-interacting protein 1 in the detection, sequestration and exocytosis of senescent mitochondria (PubMed : 37681898).

See full target information PKD1

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomedicines 13: PubMed40299470

2025

Polycystins Expression in Astrocytic Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Martha Assimakopoulou,Konstantina Soufli,Maria Melachrinou

Oncogenesis 12:31 PubMed37244923

2023

Integrated analysis of single-cell and bulk RNA sequencing data reveals an immunostimulatory microenvironment in tumor thrombus of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ji,Qianyu Shi,Song Mei,Jiuhui Xu,Haijie Liang,Lu Xie,Tingting Ren,Kunkun Sun,Dasen Li,Xiaodong Tang,Peng Zhang,Wei Guo

The Journal of biological chemistry 296:100680 PubMed33872598

2021

Nonredundant roles of DIAPHs in primary ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Oliva Palander,Adam Lam,Richard F Collins,Theo J Moraes,William S Trimble

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Polycystin 1/PC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 1/PC1 antibody (AB235963)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody (AB235963)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise