AB201793

Anti-PORCN/PPN antibody [EPR19741]

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PORCN/PPN antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

MG61, PORC, PPN, PORCN, Protein-serine O-palmitoleoyltransferase porcupine, Protein MG61

1 이미지

Supplier Data

Western blot - Anti-PORCN/PPN antibody [EPR19741] (AB201793)

Blocking/Dilution buffer : 5% NFDM/TBST.

Our immunogen covers all 5 isoforms but only isoform 5 (42kDa) is observed in our testing condition. This is consistent with the literature (PMID : 21768372).

All lanes:

Western blot - Anti-PORCN/PPN antibody [EPR19741] (ab201793) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 42 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19741

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16 : 1(9Z)), to Wnt proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.

See full target information PORCN

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Mediators of inflammation 2025:3585885 PubMed40726812

2025

G Protein-Coupled Receptor 30 Attenuates Neuronal Pyroptosis Induced by Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Peng,Xiqi Hu,Jun He,Ying Xia

Translational cancer research 12:1703-1714 PubMed37588740

2023

PORCN promotes hepatocellular carcinogenesis via Wnt/β-catenin-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhen Wu,Zhaoxiu Liu,Jing Chen,Tao Ma,Liyang Wang,Tianxin Huang,Yu Sheng,Wei Huang,Cuihua Lu

Nature cell biology 23:953-966 PubMed34475534

2021

Release of Notch activity coordinated by IL-1β signalling confers differentiation plasticity of airway progenitors via Fosl2 during alveolar regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwook Choi,Yu Jin Jang,Catherine Dabrowska,Elhadi Iich,Kelly V Evans,Helen Hall,Sam M Janes,Benjamin D Simons,Bon-Kyoung Koo,Jonghwan Kim,Joo-Hyeon Lee

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PORCN/PPN antibody [EPR19741]

Western blot - Anti-PORCN/PPN antibody [EPR19741] (AB201793)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise