AB327242

Anti-POU3F2 Antibody

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Rabbit Polyclonal Brn-2 antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2.

대체 명칭 보기

BRN2, OCT7, OTF7, POU3F2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7

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Immunocytochemistry/ Immunofluorescence - Anti-POU3F2 Antibody (AB327242)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POU3F2 Antibody (AB327242)

Immunofluorescent staining of human cell line AF22 using ab327242 at 0.25–2 µg/ml, showing localization to nucleus.

ChIP

Supplier Data

ChIP - Anti-POU3F2 Antibody (AB327242)

ChIP-Exo-Seq composite graph for ab327242 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human POU3F2.

P20265

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

See full target information POU3F2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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