AB327159

Anti-POU3F3 Antibody [CL15302]

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Mouse Monoclonal POU3F3/Brain1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human POU3F3.

대체 명칭 보기

BRN1, OTF8, POU3F3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8, Brain-1, Brn-1, Oct-8, OTF-8

11 이미지

Immunocytochemistry/ Immunofluorescence - Anti-POU3F3 Antibody [CL15302] (AB327159)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunofluorescence staining in SH-SY5Y cell line using ab327159 at 2–10 µg/ml, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human kidney using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in cells in tubules.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human cerebral cortex using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in neurons and glial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327159 at 1 : 1000–1 : 2500 dilution. Corresponding POU3F3 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of rat cerebellum using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in a subset of neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of mouse cerebral cortex using ab327159 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of rat hippocampus using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in a subset of neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of mouse cerebellum using ab327159 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in a subset of neurons.

Supplier Data

Western blot - Anti-POU3F3 Antibody [CL15302] (AB327159)

Western blot analysis in human cell line HEK 293 using ab327159 at 1 µg/ml.

All lanes:

Western blot - Anti-POU3F3 Antibody [CL15302] (ab327159)

All lanes:

Human cell line HEK 293

Predicted band size: 50.33 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human liver using ab327159 at 1 : 1000–1 : 2500 dilution, showing no positivity in hepatocytes as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human skeletal muscle using ab327159 at 1 : 1000–1 : 2500 dilution, showing no positivity in myocytes as expected.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL15302

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human POU3F3.

P20264

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development (PubMed : 31303265). Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).

See full target information POU3F3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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