Anti-PP1C gamma antibody [EPR8934]

• WB

Lab

Western blot - Anti-PP1C gamma antibody [EPR8934] (AB134947)

Lanes 1-4 : Merged signal (red and green). Green - ab134947 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.

ab134947 Anti-Protein phosphatase 1 gamma 2 antibody was shown to specifically react with Protein phosphatase 1 gamma 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264875 (knockout cell lysate ab258131) was used. Wild-type and Protein phosphatase 1 gamma 2 knockout samples were subjected to SDS-PAGE. ab134947 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPP1CC knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPP1CC (PP1C gamma) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-ppp1cc-pp1c-gamma-knockout-hela-cell-line-ab264875'>ab264875</a>)

Lane 3:

T-47D cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa,37 kDa,42 kDa

Observed band size: 28 kDa,37 kDa,38 kDa

false

• WB

Unknown

Western blot - Anti-PP1C gamma antibody [EPR8934] (AB134947)

All lanes:

Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947) at 1/1000 dilution

Lane 1:

T47D cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SKBR3 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

true

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PP1C gamma antibody [EPR8934] (AB134947)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.