Anti-PP2A alpha + beta antibody [YE351]
- 20ul selling size
- RabMAb
- Recombinant
- 각 태그의 뜻
5
(1 리뷰)
|
(8 제품이 사용된 논문 )
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasm and weak nucleus staining is visible in lymphocytes of human tonsil.
- IP
Lab
Immunoprecipitation - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
ab32065 at 1/80 immunoprecipitating PP2A alpha + beta in HeLa whole cell lysate.
Lane 1 (input) : HeLa whole cell lysate (10μg)
Lane 2 (+) : ab32065 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32065 in HeLa (human cervix adenocarcinoma) whole cell lysate.
For western blotting, ab32065 (1/500) ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000).
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
Observed band : 37kDa.
All lanes:
Immunoprecipitation - Anti-PP2A alpha + beta antibody [YE351] (ab32065)
Predicted band size: 36 kDa
false
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat heart tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasm and nucleus staining is visible on rat heart.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasm and weak nucleus staining is visible in epithelial cells of rat kidney.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasm staining is visible in mouse heart.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasm staining is visible in epithelial cells of mouse kidney
- WB
Lab
Western blot - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/50000 dilution
Lane 1:
Jurkat whole cell lysate at 10 µg
Lane 2:
U-937 whole cell lysate at 10 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 5s
- WB
Lab
Western blot - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/100000 dilution
Lane 1:
Human fetal heart tissue lysate at 10 µg
Lane 2:
Human fetal kidney tissue lysate at 10 µg
Lane 3:
Human fetal spleen tissue lysate at 10 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 30s
- WB
Lab
Western blot - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Blocking and dilution buffer : 5% NFDM /TBST.
All lanes:
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/100000 dilution
All lanes:
Human fetal brain tissue lysate at 10 µg
Secondary
All lanes:
HRP-conjugate anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 15s
- WB
Lab
Western blot - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/50000 dilution
Lane 1:
A431 whole cell lysate at 10 µg
Lane 2:
A549 whole cell lysate at 10 µg
Lane 3:
Caco-2 whole cell lysate at 10 µg
Lane 4:
T47D whole cell lysate at 10 µg
Lane 5:
C2C12 whole cell lysate at 10 µg
Lane 6:
HeLa whole cell lysate at 10 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 15s
- WB
Lab
Western blot - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Blocking and dilution buffer : 5% NFDM /TBST.
All lanes:
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/100000 dilution
Lane 1:
Mouse brain tissue lysate at 10 µg
Lane 2:
Mouse heart tissue lysate at 10 µg
Lane 3:
Mouse kidney tissue lysate at 10 µg
Lane 4:
Mouse spleen tissue lysate at 10 µg
Lane 5:
Rat brain tissue lysate at 10 µg
Lane 6:
Rat heart tissue lysate at 10 µg
Lane 7:
Rat kidney tissue lysate at 10 µg
Lane 8:
Rat spleen tissue lysate at 10 µg
Lane 9:
C6 whole cell lysate at 10 µg
Lane 10:
Raw264.7 whole cell lysate at 10 µg
Lane 11:
PC-12 whole cell lysate at 10 µg
Lane 12:
NIH/3T3 whole cell lysate at 10 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
Exposure time: 1s
- Dot
Unknown
Dot Blot - Anti-PP2A alpha + beta antibody [YE351] (AB32065)
Dot blot analysis of PP2A-alpha peptide (Lane 1) and PP2A-beta peptide (Lane 2) labelling PP2A alpha+beta with ab32065 at a dilution of 1/1000. A HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at a dilution of 1/1000.
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
관련 conjugated 항체와 다양한 조성의 항체 (1)
-
Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free
Reactivity 정보
제품 세부 정보
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
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보관 버퍼
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적절한 단기 보관 조건
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타겟 정보
Additional targets
대체 명칭 보기
제품이 사용된 논문 (8)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404080 PubMed39041921
2024
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Regenerative therapy 21:560-573 PubMed36475023
2022
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Cell death discovery 8:368 PubMed35977927
2022
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Molecular therapy. Nucleic acids 26:280-294 PubMed34513310
2021
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American journal of physiology. Gastrointestinal a 317:G408-G428 PubMed31393787
2019
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Life sciences 213:166-173 PubMed30340029
2018
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Journal of neuropathology and experimental neurology 77:139-148 PubMed29281045
2017
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PloS one 5:e11584 PubMed20644732
2010
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WB
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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