AB249330

Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PPP2R2B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.

대체 명칭 보기

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B

2 이미지

Lab

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

This data was developed using ab157461, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3.25 seconds.

Recombinant Human PPP2R2B protein (Cat

: ab132644).

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (<a href='/ko/products/primary-antibodies/ppp2r2b-antibody-epr10961-ab157461'>ab157461</a>) at 1/1000 dilution

All lanes:

GST-tagged Recombinant Human PPP2R2B protein (aa 1 to 443) 15ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 76 kDa

false

Unknown

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

This data was developed using ab157461, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (<a href='/ko/products/primary-antibodies/ppp2r2b-antibody-epr10961-ab157461'>ab157461</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10961

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody may also recognize PPP2R2A and PPP2R2D based on our internal QC data.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab249330 is the carrier-free version of ab157461.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 2 regulatory subunit B also known as PPP2R2B or B beta regulatory subunit plays an important role in cellular processes. It has a molecular mass of approximately 55 kDa. This subunit is part of the protein phosphatase 2 (PP2A) complex and is expressed widely but found at high levels in the brain. In particular its expression is prominent in specific regions such as the cerebellum.

Biological function summary

PPP2R2B acts as a regulatory component of the PP2A holoenzyme complex. PP2A is a serine/threonine phosphatase involved in the negative control of cell growth and division. PPP2R2B is one of the regulatory subunits that directs the PP2A core enzyme to certain substrates influencing dephosphorylation activities. It participates in maintaining normal cellular functions especially within the nervous system by modulating the phosphorylation state of various proteins.

Pathways

PPP2R2B impacts the cell cycle and PI3K/AKT signaling pathways. It negatively regulates these pathways by dephosphorylating target proteins acting as a tumor suppressor. The regulation by PPP2R2B of the PI3K/AKT pathway links it to controlling cell proliferation and survival interacting with proteins like AKT1 and mTOR. The involvement of PPP2R2B in these pathways reflects its pivotal role in ensuring cellular homeostasis and response to extracellular signals.

PPP2R2B is associated with neurodegenerative conditions including spinocerebellar ataxia type 12 and Huntington’s disease. Abnormalities in PPP2R2B function or expression can disrupt normal neuronal activities leading to these disorders. In Huntington’s disease alterations in PPP2R2B contribute to the pathology by affecting the dephosphorylation dynamics of proteins such as huntingtin exacerbating disease symptoms and progression. Understanding the modulation of PPP2R2B in diseases can offer insights into potential therapeutic targets for these debilitating conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance (By similarity).

See full target information PPP2R2B

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Assay Kits

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise