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AB316368

Anti-PPP2R5D antibody [H5D12]

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Mouse Monoclonal PPP2R5D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform.

대체 명칭 보기

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform R5-delta, PPP2R5D

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5D Antibody [H5D12] (AB316368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5D Antibody [H5D12] (AB316368)

Immunofluorescence analysis of paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Western blot - Anti-PPP2R5D Antibody [H5D12] (AB316368)
  • WB

Supplier Data

Western blot - Anti-PPP2R5D Antibody [H5D12] (AB316368)

(1 µg/ml) staining in HeLa (A), (0.1 µg/ml) staining in MCF7 (B), A431 (C) and (0.03 µg/ml) staining in Jurkat (D) cells lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Lane 1:

Western blot - Anti-PPP2R5D Antibody [H5D12] (ab316368) at 1 µg/mL

Lane 1:

HeLa cell lysate

Lanes 2 - 3:

Western blot - Anti-PPP2R5D Antibody [H5D12] (ab316368) at 0.1 µg/mL

Lane 2:

MCF7 cell lysate

Lane 3:

A431 cell lysate

Lane 4:

Western blot - Anti-PPP2R5D Antibody [H5D12] (ab316368) at 0.03 µg/mL

Lane 4:

Jurkat cell lysate

true

Exposure time: 1hr

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

H5-D12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform.

Q14738

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보완 제품

Primary Antibodies

AB32444

Anti-Cdc25C antibody [E302]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody [E302] (AB32444)

  • 4
  • 3
Primary Antibodies

AB32065

Anti-PP2A alpha + beta antibody [YE351]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (AB32065)

  • 5
  • 1
Primary Antibodies

AB198500

Anti-PPP2R5E antibody [EPR17147] - C-terminal

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (AB198500)

  • 0
  • 0
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
보관 버퍼
Preservative: 0.1% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1 month
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R5D also known as B56δ forms part of the regulatory subunit of protein phosphatase 2A (PP2A). Protein phosphatase 2A is an important enzyme in cellular signaling that dephosphorylates target proteins. The approximate molecular mass of PPP2R5D is 72 kDa. It is found expressed in various tissues throughout the human body but its levels can vary between different cell types.
Biological function summary

PPP2R5D plays an essential role in the regulation of cell growth and division. This protein is part of the PP2A holoenzyme complex which assembles with catalytic and scaffolding subunits. Through its regulatory influence PPP2R5D affects the phosphorylation state of several substrates thereby impacting cellular processes like cell cycle progression and apoptosis.

Pathways

PPP2R5D significantly contributes to the MAPK signaling pathway and the Wnt pathway. It modulates phosphorylation states affecting signal transduction mechanisms. In the MAPK pathway PPP2R5D interacts with proteins like ERK1/2 by influencing their activity. Similarly in the Wnt pathway it acts on components important for maintaining cellular balance and signaling fidelity.

PPP2R5D mutations and dysregulation have been linked to intellectual disabilities and cancer. Changes in its function are associated with Autism Spectrum Disorder where it potentially interacts with other proteins involved in neuronal development. In cancer altered PPP2R5D activity may disrupt normal cellular growth control often involving proteins like c-Myc that are critical for proliferation and survival.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.
See full target information Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
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Product promise

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