Anti-PRDM1/Blimp1 antibody [ROS195G]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [ROS195G] (AB241568)

ab241568 staining PRDM1/Blimp1 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab241568 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• WB

Lab

Western blot - Anti-PRDM1/Blimp1 antibody [ROS195G] (AB241568)

Lanes 1 - 9 : Merged signal (red and green). Green - ab241568 observed at 85 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

Lanes 7 - 9 : Increased green channel intensity of lanes 4 - 6.

ab241568 was shown to react with PRDM1/Blimp1 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab241568 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PRDM1/Blimp1 antibody [ROS195G] (ab241568) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lanes 4 and 7:

Raji whole cell lysate at 20 µg

Lanes 5 and 8:

Human PTA-6967 whole cell lysate at 20 µg

Lanes 6 and 9:

A431 whole cell lysate at 20 µg

Predicted band size: 91 kDa

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