AB233759

Anti-PRDM4/PFM1 antibody [4B6G1]

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Mouse Monoclonal PRDM4/PFM1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PRDM4 aa 450-600.

대체 명칭 보기

PFM1, PRDM4, PR domain zinc finger protein 4, PR domain-containing protein 4

3 이미지

Flow Cytometry - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PRDM4/PFM1 with ab233759 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

All lanes:

Western blot - Anti-PRDM4/PFM1 antibody [4B6G1] (ab233759) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

PRDM4/PFM1 (AA: 476-575)-hIgGFc transfected HEK-293T whole cell lysate

Predicted band size: 88 kDa

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Supplier Data

Western blot - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

All lanes:

Western blot - Anti-PRDM4/PFM1 antibody [4B6G1] (ab233759) at 1/500 dilution

All lanes:

Recombinant human PRDM4/PFM1 (AA: 476-575) protein

Predicted band size: 88 kDa

true

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

4B6G1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human PRDM4 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q9UKN5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PRDM4 also known as PFM1 or PFM-1 is a member of the PR domain-containing protein family. It possesses an approximate molecular weight of 75 kDa. This protein functions primarily as a transcription factor with roles in the regulation of gene expression. PRDM4 localizes mainly in the nucleus where it influences transcriptional control. Expression of PRDM4/PFM1 has been observed in various tissues but shows significant levels in the brain and reproductive organs indicating its involvement in important biological processes in these regions.

Biological function summary

PRDM4/PFM1 acts in cell differentiation and development. It is part of the KRAB-ZnF silencing complex which connects to other components to modify chromatin structure and regulate gene expression. This activity is important to maintaining cellular functions and contributes to cellular differentiation states. Understanding PRDM4/PFM1’s role within this complex can provide insights into its broader biological actions and its influence on cellular dynamics.

Pathways

PRDM4/PFM1 plays a significant role in the TGF-beta and Hedgehog signaling pathways. These pathways are major determinants of cellular growth differentiation and apoptosis. Within these pathways PRDM4/PFM1 can associate with other proteins like SMAD family members and GLI transcription factors which are important in mediating cellular responses to external signals.

PRDM4/PFM1 links to neurodegenerative conditions and certain cancers. Its dysregulation has been noted in glioblastoma where an altered expression pattern suggests its involvement in tumor progression. PRDM4/PFM1 interacts with proteins involved in these disorders such as PTEN—a known tumor suppressor gene—highlighting its potential role in the development and progression of these diseases. Understanding these connections is key for developing therapeutic strategies targeting PRDM4/PFM1-related pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May function as a transcription factor involved in cell differentiation.

See full target information PRDM4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC musculoskeletal disorders 25:1081 PubMed39736635

2024

The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Wang,Zhengwei Duan,Zihua Li,Dong Yang,Hengli Lu,Yiwei Zhang,Yuesong Fu,Yonghao Guan,Guodong Li,Feng Qian,Tianyang Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRDM4/PFM1 antibody [4B6G1]

Flow Cytometry - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

Western blot - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

Western blot - Anti-PRDM4/PFM1 antibody [4B6G1] (AB233759)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise