AB61409

Anti-Prion protein PrP antibody [8H4]

Name: Anti-Prion protein PrP antibody [8H4]
Brand: Abcam Limited
SKU: AB61409
Rating: 3 (3 reviews)

(3 리뷰들)

(17 제품이 사용된 논문 )

Anti-Prion protein PrP antibody [8H4] (ab61409) is a mouse monoclonal antibody detecting Prion protein PrP in Western Blot, Flow Cytometry, IP, IHC-P, ELISA. Suitable for Cow, Human, Monkey, Mouse, Rat, Sheep.

- Over 10 publications
- Trusted since 2007

1 이미지

AbReview45016****

Western blot - Anti-Prion protein PrP antibody [8H4] (AB61409)

All lanes:

Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409) at 1/2500 dilution

Lane 1:

Wild type mouse hippocampus whole tissue lysate at 30 µg

Lane 2:

Prnp-/- mouse hippocampus whole tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated sheep anti-mouse IgG monoclonal at 1/5000 dilution

Predicted band size: 28 kDa

Observed band size: 25-37 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous Abreview

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

8H4

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Sheep, Monkey, Human, Cow, Rat

Applications

I-ELISA, WB, IP, ICC, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Prnp.

P04925

Epitope

The antibody epitope resides within amino acids 145-180 of human Prion protein PrP.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Cow": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Sheep": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

style

left-column

제품 세부 정보

What is this antibody validated in?
Anti-Prion protein PrP antibody [8H4] (ab61409) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA in Cow, Human, Monkey, Mouse, Rat, Sheep samples.

Trusted by the scientific community
Anti-Prion protein PrP [8H4] (ab61409) was first used in a scientific publication in 2007 and has been cited over 10 times in peer-reviewed journals.

style

left-column

특성 및 보관 정보

제형

Liquid

Purity

IgG fraction

Purification 관련 사항

Purified Immunoglobulin

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.

Biological function summary

Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.

Pathways

Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.

Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

See full target information PRNP

대체 명칭 보기

CD230, ALTPRP, PRIP, PRP, PRNP, Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C

style

left-column

제품이 사용된 논문 (17)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature medicine 31:1319-1328 PubMed39810005

2025

In vivo base editing extends lifespan of a humanized mouse model of prion disease.

Applications

Unspecified application

Species

Unspecified reactive species

Meirui An,Jessie R Davis,Jonathan M Levy,Fiona E Serack,John W Harvey,Pamela P Brauer,Catherine P Pirtle,Kiara N Berríos,Gregory A Newby,Wei-Hsi Yeh,Nikita Kamath,Meredith Mortberg,Yuan Lian,Michael Howard,Kendrick DeSouza-Lenz,Kenia Guzman,Aaron Thai,Samantha Graffam,Vanessa Laversenne,Alissa A Coffey,Jeannine Frei,Sarah E Pierce,Jiri G Safar,Benjamin E Deverman,Eric Vallabh Minikel,Sonia M Vallabh,David R Liu

Science (New York, N.Y.) 384:ado7082 PubMed38935715

2024

Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic editor.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin N Neumann,Tessa M Bertozzi,Elaine Wu,Fiona Serack,John W Harvey,Pamela P Brauer,Catherine P Pirtle,Alissa Coffey,Michael Howard,Nikita Kamath,Kenney Lenz,Kenia Guzman,Michael H Raymond,Ahmad S Khalil,Benjamin E Deverman,Eric Vallabh Minikel,Sonia M Vallabh,Jonathan S Weissman

Nature communications 14:8131 PubMed38065962

2023

Excess PrP inhibits muscle cell differentiation via miRNA-enhanced liquid-liquid phase separation implicated in myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Tao,Yanping Zeng,Bin Dai,Yin Liu,Xiaohan Pan,Li-Qiang Wang,Jie Chen,Yu Zhou,Zuneng Lu,Liwei Xie,Yi Liang

Alzheimer's research & therapy 15:201 PubMed37968719

2023

Neuronal transcriptome, tau and synapse loss in Alzheimer's knock-in mice require prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Austin Stoner,Li Fu,LaShae Nicholson,Chao Zheng,Takuya Toyonaga,Joshua Spurrier,Will Laird,Zhengxin Cai,Stephen M Strittmatter

Science signaling 15:eabm3720 PubMed36378750

2022

M muscarinic receptor activation reduces the molecular pathology and slows the progression of prion-mediated neurodegenerative disease.

Applications

Unspecified application

Species

Unspecified reactive species

Louis Dwomoh,Mario Rossi,Miriam Scarpa,Elham Khajehali,Colin Molloy,Pawel Herzyk,Shailesh N Mistry,Andrew R Bottrill,Patrick M Sexton,Arthur Christopoulos,Jeffrey Conn,Craig W Lindsley,Sophie J Bradley,Andrew B Tobin

JCI insight 7: PubMed35133987

2022

Regional variability and genotypic and pharmacodynamic effects on PrP concentration in the CNS.

Applications

Unspecified application

Species

Unspecified reactive species

Meredith A Mortberg,Hien T Zhao,Andrew G Reidenbach,Juliana E Gentile,Eric Kuhn,Jill O'Moore,Patrick M Dooley,Theresa R Connors,Curt Mazur,Shona W Allen,Bianca A Trombetta,Alison McManus,Matthew R Moore,Jiewu Liu,Deborah E Cabin,Holly B Kordasiewicz,Joel Mathews,Steven E Arnold,Sonia M Vallabh,Eric Vallabh Minikel

International journal of molecular sciences 22: PubMed34445708

2021

Concussion/Mild Traumatic Brain Injury (TBI) Induces Brain Insulin Resistance: A Positron Emission Tomography (PET) Scanning Study.

Applications

Unspecified application

Species

Unspecified reactive species

Sathiya Sekar,Raja Solomon Viswas,Hajar Miranzadeh Mahabadi,Elahe Alizadeh,Humphrey Fonge,Changiz Taghibiglou

Molecular brain 14:89 PubMed34099009

2021

Cannabinoid receptors distribution in mouse cortical plasma membrane compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Hajar Miranzadeh Mahabadi,Haseeb Bhatti,Robert B Laprairie,Changiz Taghibiglou

PloS one 14:e0218509 PubMed31206560

2019

Epitope mapping of the protease resistant products of RT-QuIC does not allow the discrimination of sCJD subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Piconi,Alexander H Peden,Marcelo A Barria,Alison J E Green

Transfusion 59:2429-2435 PubMed31020675

2019

Integrative genome analysis identified the KANNO blood group antigen as prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Omae,Shoichi Ito,Mayumi Takeuchi,Kazumi Isa,Kenichi Ogasawara,Kinuyo Kawabata,Akira Oda,Sayaka Kaito,Hatsue Tsuneyama,Makoto Uchikawa,Ikuo Wada,Hitoshi Ohto,Katsushi Tokunaga

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prion protein PrP antibody [8H4]

Western blot - Anti-Prion protein PrP antibody [8H4] (AB61409)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (17)

Product promise