Anti-pro-EGF antibody [EPR14622]

• IP

Supplier Data

Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (AB206106)

EGF was immunoprecipitated from 1mg of Human milk sample with ab206106 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206106 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human milk sample 10ug (Input).

Lane 2 : ab206106 IP in Human milk sample.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206106 in Human milk sample.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (ab206106)

false

• WB

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

All lanes:

Human urine sample at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 1 second

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

Lane 1:

Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag at 0.015 µg

Lane 2:

Recombinant Human EGF recombinant protein (aa971-1023) at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

false

• WB

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human EGF full length protein contains aa21-1023 with His-Tag® at the C-Terminus.

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/5000 dilution

All lanes:

Human EGF recombinant protein at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

All lanes:

Human milk sample at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

false

Exposure time: 3min