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AB281141

Anti-Pro-MCH antibody [EPR23778-68] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal MCH antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

MCH, PMCH, Pro-MCH

1 이미지
Sandwich ELISA - Anti-Pro-MCH antibody [EPR23778-68] - BSA and Azide free (Capture) (AB281141)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Pro-MCH antibody [EPR23778-68] - BSA and Azide free (Capture) (AB281141)

Example of human Pro-MCH standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23778-68

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB303757

Human Pro-MCH ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-MCH ELISA Kit (AB303757)

  • 0
  • 0
Primary Antibodies

AB6214

Anti-Orexin A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (AB6214)

  • 5
  • 2
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB269380

Anti-Lysozyme antibody [EPR22270-53] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Lysozyme antibody [EPR22270-53] - BSA and Azide free (Capture) (AB269380)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab281141 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PMCH. The recommended pair for sandwich ELISA is:
Capture: ab281141, Human PMCH Capture Antibody (unconjugated)
Detector: ab280991, Human PMCH Detector Antibody (unconjugated)
The reference range value is 39 - 10000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pro-MCH also known as pro-melanin-concentrating hormone is a precursor polypeptide that undergoes post-translational processing to produce mature melanin-concentrating hormone (MCH). The precursor weighs approximately 18 kDa before processing. Pro-MCH is expressed mainly in the hypothalamus but can also be found in other brain regions involved in regulating appetite and energy homeostasis.
Biological function summary

The pro-MCH precursor undergoes cleavage to generate MCH which plays a significant role in energy balance and feeding behavior. MCH functions are often linked to neuronal populations in the hypothalamus that regulate hunger signals and it is involved in forming neuronal circuits that influence behavior related to food intake. Although pro-MCH itself is not part of a larger complex the derived MCH interacts with its receptors to transmit signals.

Pathways

Melanin-concentrating hormone interacts with energy homeostasis and reward circuits in the brain. It is engaged in the melanocortin pathway which balances energy intake and expenditure. MCH modulates signaling by interacting with receptors such as MCHR1 and MCHR2 influencing food-seeking behavior and energy regulation processes. Additionally its regulation aligns with orexigenic (appetite-increasing) peptides such as neuropeptide Y (NPY) coordinating energy-related responses.

Aberrant MCH signaling relates to metabolic disorders such as obesity and altered response to stress. Individuals with irregular MCH signaling may exhibit increased appetite and weight gain contributing to metabolic syndrome. The interaction with MCHR1 is significant here as alterations in this receptor are associated with such metabolic changes. Furthermore pro-MCH and its derivative peptides have also been linked to mood disorders with disrupted MCH pathways influencing depression-related behaviors through interactions with proteins like corticotropin-releasing hormone (CRH).
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
See full target information PMCH
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Product promise

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