AB242369

Anti-Profilin 1 antibody [CL3524]

Lab Essentials
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Mouse Monoclonal Profilin 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFN1 aa 50 to C-terminus.

대체 명칭 보기

Profilin-1, Epididymis tissue protein Li 184a, Profilin I, PFN1

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (AB242369)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (AB242369)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Profilin 1 with ab242369 at a 1 : 1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (AB242369)

Formalin-fixed, paraffin-embedded human testis tissue stained for Profilin 1 with ab242369 at a 1 : 1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (AB242369)

Formalin-fixed, paraffin-embedded human liver tissue stained for Profilin 1 with ab242369 at a 1 : 1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (AB242369)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for Profilin 1 with ab242369 at a 1 : 1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (AB242369)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Profilin 1 with ab242369 at a 1 : 1000 dilution in immunohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3524

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PFN1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07737

style

left-column

대체품

Primary Antibodies

AB203146

Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304] (AB203146)

Primary Antibodies

AB124904

Anti-Profilin 1 antibody [EPR6304]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Profilin 1 antibody [EPR6304] (AB124904)

Primary Antibodies

AB50667

Anti-Profilin 1 antibody - Carboxyterminal end

Lab Essentials

Immunoprecipitation - Anti-Profilin 1 antibody - Carboxyterminal end (AB50667)

Primary Antibodies

AB133529

Anti-Profilin 1 antibody [EPR6303]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (AB133529)

보완 제품

Assay Kits

AB314851

Human Profilin 1 ELISA Kit

SimpleStep

Sandwich ELISA - Human Profilin 1 ELISA Kit (AB314851)

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

Primary Antibodies

AB242146

Anti-Optineurin antibody [EPR23059-124]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Optineurin antibody [EPR23059-124] (AB242146)

Primary Antibodies

AB40676

Anti-NAK/TBK1 antibody [EP611Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-NAK/TBK1 antibody [EP611Y] (AB40676)

Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Profilin 1 also known as Profilin-1 or Profilin is a small actin-binding protein with a molecular weight of approximately 15 kDa. It is expressed across various tissues including muscle brain and epithelial cells. Profilin 1 interacts with monomeric actin (G-actin) to regulate actin polymerization by binding to actin monomers. This protein also interacts with polyphosphoinositides and proline-rich sequences playing a mechanical role in balancing actin filament assembly and disassembly.

Biological function summary

Profilin 1 acts as an important regulator of the cytoskeleton facilitating cellular processes like motility and division. It participates in the formation of actin filaments by delivering actin monomers to barbed ends promoting polymerization. Profilin 1 is part of the complex that controls actin dynamics and this ability to modulate actin structure allows it to influence cell shape and internal movement. The protein's interactions help support the actin cytoskeleton for intracellular trafficking and signal transduction.

Pathways

Profilin 1 has important roles in the regulation of the actin cytoskeleton and Rho signaling pathways. These pathways are important in cell migration and adhesion. During cellular processes Profilin 1's interactions with actin and polyphosphoinositides link it to other proteins such as Rho GTPases which are associated with cytoskeletal dynamics. Profilin 1 can influence these pathways by modulating actin dynamics and interacting with proteins involved in these signaling cascades.

Disruptions in Profilin 1 function are linked to neurodegenerative diseases and cancer metastasis. For example aberrant Profilin 1 activity can contribute to amyotrophic lateral sclerosis (ALS) by affecting neuronal structure and transport. Dysregulation of its interaction with actin or polyphosphoinositides might also lead to enhanced cancer cell migration and invasion. It may interact with other proteins implicated in these diseases such as the ALS-related protein TDP-43 or cancer-related kinases making it significant in understanding these conditions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

See full target information PFN1

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 300:105583 PubMed38141770

2023

Actin-binding protein profilin1 is an important determinant of cellular phosphoinositide control.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan M C Ricci,Andrew Orenberg,Lee Ohayon,David Gau,Rachel C Wills,Yongho Bae,Tuhin Das,David Koes,Gerald R V Hammond,Partha Roy

F1000Research 12:348 PubMed37576538

2023

The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence.

Applications

Species

Human

Riham Ayoubi,Ian McDowell,Maryam Fotouhi,Kathleen Southern,Peter S McPherson,Carl Laflamme

Nucleic acids research 49:8024-8036 PubMed34223906

2021

LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ni,Kathrin Muegge

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com