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AB259664

Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Granulin antibody. Propeptide domain. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) (AB259664)
  • sELISA

Unknown

Sandwich ELISA - Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) (AB259664)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab252364).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22103-42

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB252364

Human Progranulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

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Sample Prep & Detection Kits

AB102903

PE/Cy7® Conjugation Kit - Lightning-Link®

Conjugation - PE/Cy7® Conjugation Kit - Lightning-Link® (AB102903)

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Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

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Primary Antibodies

AB256487

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

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Primary Antibodies

AB259752

Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector) (AB259752)

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Primary Antibodies

AB290688

Anti-Aquaporin 4 antibody [EPR24281-105] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Aquaporin 4 antibody [EPR24281-105] - BSA and Azide free (Capture) (AB290688)

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Primary Antibodies

AB230261

Anti-RAB10 (phospho T73) antibody [MJF-R21]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab259664 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Progranulin. The recommended pair for sandwich ELISA is:
Capture: ab259663, Human Progranulin Capture Antibody (unconjugated)
Detector: ab259664, Human Progranulin Detector Antibody (unconjugated)
The reference range value is 156.25 - 10000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Progranulin also known as Granulin or Proepithelin is a secreted glycoprotein with a molecular mass of approximately 68 kDa. It is expressed in a variety of tissues including the brain skin immune cells and certain epithelial cells. The protein consists of multiple cysteine-rich granulin modules which are important for its function. Progranulin plays a mechanical role in cell cycle regulation and wound healing by influencing cell proliferation and migration.
Biological function summary

This protein influences numerous cellular activities. Progranulin acts as a growth factor promoting cell survival and tissue repair. It is also a part of the lysosomal function where it helps to regulate lysosomal dynamics and turnover. It interacts with the receptor sortilin which mediates its uptake and function underlining its importance in maintaining cellular homeostasis.

Pathways

Progranulin participates in the tumor necrosis factor (TNF) pathway and the lysosome acidification pathway. It modulates TNF signaling by interacting with TNF receptors potentially balancing inflammatory responses. In the context of lysosomal pathways progranulin works closely with proteins like cathepsin D to facilitate lysosomal enzyme activity and stability ensuring effective cellular turnover and waste management.

This protein has significant implications in neurodegenerative disorders and cancer. Mutations or alterations in progranulin levels associate with frontotemporal lobar degeneration (FTLD) a form of dementia. Reduced progranulin levels contribute to neuroinflammation and neuronal loss in FTLD. In cancer progranulin is linked to tumor growth and progression interacting with proteins involved in cell proliferation and survival such as transforming growth factor-beta (TGF-beta). Understanding these connections highlights its potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12526812, PubMed : 18378771, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286). Regulates protein trafficking to lysosomes, and also the activity of lysosomal enzymes (PubMed : 28453791, PubMed : 28541286). Also facilitates the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 12526812). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 18378771).. Granulin-4. Promotes proliferation of the epithelial cell line A431 in culture.. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
See full target information GRN

대체 명칭 보기

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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