AB254276

Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal Properdin/PFC antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Mouse, Rat, Human samples.

대체 명칭 보기

PFC, CFP, Properdin, Complement factor P

3 이미지

Indirect ELISA - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

This data was developed using ab252532, the same antibody clone in a different buffer formulation.

Indirect ELISA antibody dose-response curve using properdin at 1000 ng/ml and ab252532 at 0-2000 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution.

Lab

Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

This data was developed using ab252532, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24797388).

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-Properdin/PFC antibody [6E9E6] (<a href='/ko/products/primary-antibodies/properdin-pfc-antibody-6e9e6-ab252532'>ab252532</a>) at 1/500 dilution

All lanes:

Human plasma lysate 20ug

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/ko/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

This data was developed using ab252532, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24797388).

Exposure time : 70 seconds.

All lanes:

Western blot - Anti-Properdin/PFC antibody [6E9E6] (<a href='/ko/products/primary-antibodies/properdin-pfc-antibody-6e9e6-ab252532'>ab252532</a>) at 1/500 dilution

Lane 1:

Rat serum lysate 20ug

Lane 2:

Mouse plasma lysate 20ug

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/ko/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 55 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

6E9E6

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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제품 세부 정보

ab254276 is the carrier-free version of ab252532.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Properdin also known as factor P or complement factor P plays an essential role in the immune system. It is a serum protein with a molecular mass of approximately 53 kDa. Properdin is most commonly expressed in monocytes neutrophils and activated macrophages. It serves as a positive regulator in the alternative pathway of the complement system providing stability to the complement convertase complexes C3bBb and C3bBbC3b.

Biological function summary

Properdin enhances the activity of the complement system by stabilizing the C3 and C5 convertase enzyme complexes. These complexes are critical in the amplification of the complement cascade leading to pathogen opsonization and lysis. Properdin does not form part of a complex alone but helps existing complement complexes carry out their functions more effectively. By supporting these complexes properdin contributes to immune surveillance and the host's response to microbial invaders.

Pathways

Properdin operates within the alternative complement pathway and plays a pivotal role in immune defense. It interacts with other components such as C3 and C5 contributing to the pathway's ability to clear pathogens and damaged cells. Properdin's activity also indirectly influences other related proteins in the complement system supporting a coordinated immune response that involves factors beyond the alternative pathway itself.

Properdin deficiencies or dysregulations can lead to increased susceptibility to infections particularly by Neisseria species accounting for recurrent meningococcal disease. Additionally abnormal properdin function links to autoimmune conditions such as systemic lupus erythematosus. This connection is due to its relationship with various complement factors such as C3 which are vital for maintaining immune homeostasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

A positive regulator of the alternate pathway (AP) of complement (PubMed : 16301317, PubMed : 20382442, PubMed : 28264884, PubMed : 9748277). It binds to and stabilizes the C3- and C5-convertase enzyme complexes (PubMed : 16301317, PubMed : 20382442, PubMed : 28264884, PubMed : 9748277). Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b) (PubMed : 31507604).

See full target information CFP

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free

Indirect ELISA - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

Western blot - Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (AB254276)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise