AB180589

Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765]

Name: Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765]
Brand: Abcam Limited
SKU: AB180589
Rating: 3 (1 reviews)

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(10 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Prostaglandin E Synthase/MPGES-1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 10 publications.

3 이미지

Supplier Data

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (AB180589)

All lanes:

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589) at 1/1000 dilution

Lane 1:

NIH 3T3 cell line lysate at 20 µg

Lane 2:

Jurkat cell line lysate at 20 µg

Lane 3:

HeLa cell line lysate at 20 µg

Lane 4:

A549 cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (AB180589)

All lanes:

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589) at 1/1000 dilution

Lane 1:

Lysate of NIH 3T3 cells at 10 µg with Immunizing peptide

Lane 2:

Lysate of NIH 3T3 cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 13 kDa

false

CiteAb

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (AB180589)

Prostaglandin E Synthase/MPGES-1 western blot using anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] ab180589. Publication image and figure legend from Monteleone, N. J. & Lutz, C. S., 2020, Oncotarget, PubMed 32655834.

ab180589 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180589 please see the product overview.

The AA pathway is regulated by miR-708 in lung cancer cells.(A) Western blot analysis of proteins from A549 cells that were transiently transfected with 12.5 nM miR-708, 25 nM miR-708, or 25 nM NC miR for 48 hours. GAPDH served as a loading control, and FLAP served as a negative control. (B) Quantification of western bot from (A), n = ≥ 3. (C) PGE2 ELISA of supernatant collected from mock, NC miR, and miR-708 treated A549 cells. Data were normalized to total protein. GAPDH served as a loading control. All western blots are representative of 3 independent experiments. (***) p < .001, (****) p < .0001, n ≥ 3.

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13765

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Synthase (MPGES-1) also known as prostin E is a membrane-associated protein that catalyzes the conversion of prostaglandin H2 to prostaglandin E2. It has a molecular mass of about 16 kDa. MPGES-1 is widely expressed in various tissues including the kidneys lungs and heart. The expression of MPGES-1 increases in response to pro-inflammatory signals highlighting its adaptive response during inflammation.

Biological function summary

Prostaglandin E Synthase plays an important role in mediating inflammatory responses and is part of the terminal enzyme complex in the prostaglandin synthesis pathway. Its activity contributes to the elevated production of prostaglandin E2 (PGE2) a bioactive lipid that regulates inflammation fever and pain. MPGES-1 functions in concert with Cyclooxygenase-2 (COX-2) as part of the coordinated induction during inflammatory stimuli.

Pathways

The MPGES-1 enzyme is central to the arachidonic acid cascade specifically in the cyclooxygenase pathway. This pathway leads to the production of prostaglandins which are involved in inflammatory and immune responses. Prostaglandin E2 produced by MPGES-1 is related to proteins such as COX-2 and cytosolic phospholipase A2 (cPLA2) which precede it in the biosynthesis pathway and act collaboratively in the metabolism of arachidonic acid to prostanoids.

MPGES-1 is implicated particularly in conditions like rheumatoid arthritis and cancer. Elevated levels of prostaglandin E2 produced by the concerted action of MPGES-1 have been observed in inflamed joints of arthritis patients correlating with COX-2 expression. In cancer upregulation of MPGES-1 contributes to tumorigenesis through promoting angiogenesis and proliferation. Its interaction with COX-2 enhances oncogenic processes signifying its potential as a therapeutic target.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Terminal enzyme of the cyclooxygenase (COX)-2-mediated prostaglandin E2 (PGE2) biosynthetic pathway. Catalyzes the glutathione-dependent oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) in response to inflammatory stimuli (PubMed : 10377395, PubMed : 10869354, PubMed : 12244105, PubMed : 12460774, PubMed : 12672824, PubMed : 18682561). Plays a key role in inflammation response, fever and pain (By similarity). Also catalyzes the oxidoreduction of endocannabinoids into prostaglandin glycerol esters and PGG2 into 15-hydroperoxy-PGE2 (PubMed : 12244105, PubMed : 12672824). In addition, displays low glutathione transferase and glutathione-dependent peroxidase activities, toward 1-chloro-2,4-dinitrobenzene and 5-hydroperoxyicosatetraenoic acid (5-HPETE), respectively (PubMed : 12672824).

See full target information PTGES

대체 명칭 보기

MGST1L1, MPGES1, PGES, PIG12, PTGES, Prostaglandin E synthase, Glutathione peroxidase PTGES, Glutathione transferase PTGES, Microsomal glutathione S-transferase 1-like 1, Microsomal prostaglandin E synthase 1, p53-induced gene 12 protein, MGST1-L1, MPGES-1

style

left-column

제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Translational cancer research 14:3149-3160 PubMed40530162

2025

Ubiquitin-specific peptidase 20 affects immune cell infiltration by regulating prostaglandin E2 on intraperitoneal metastasis model of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Hua,Xiukun Ma,Xinyu Zhao,Xiaomeng Wei,Xiaojing Mu,Botao Wang,Xiangfei Yuan

Nature cell biology 26:1261-1273 PubMed38969763

2024

Functional multi-organelle units control inflammatory lipid metabolism of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Zimmermann,Kerstin Lucht,Manuel Stecher,Chahat Badhan,Katharina M Glaser,Maximilian W Epple,Lena R Koch,Ward Deboutte,Thomas Manke,Klaus Ebnet,Frauke Brinkmann,Olesja Fehler,Thomas Vogl,Ev-Marie Schuster,Anna Bremser,Joerg M Buescher,Angelika S Rambold

iScience 27:108775 PubMed38261967

2024

Influenza A virus selectively elevates prostaglandin E formation in pro-resolving macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Paul M Jordan,Kerstin Günther,Vivien Nischang,Yuping Ning,Stefanie Deinhardt-Emmer,Christina Ehrhardt,Oliver Werz

Immunology 166:47-67 PubMed35143048

2022

Staphylococcus aureus controls eicosanoid and specialized pro-resolving mediator production via lipoteichoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Miek,Paul M Jordan,Kerstin Günther,Simona Pace,Timo Beyer,David Kowalak,Verena Hoerr,Bettina Löffler,Lorena Tuchscherr,Charles N Serhan,Jana Gerstmeier,Oliver Werz

Oncoimmunology 11:2029298 PubMed35127252

2022

MUC1-C integrates type II interferon and chromatin remodeling pathways in immunosuppression of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Hagiwara,Atsushi Fushimi,Atrayee Bhattacharya,Nami Yamashita,Yoshihiro Morimoto,Mototsugu Oya,Henry G Withers,Qiang Hu,Tao Liu,Song Liu,Kwok K Wong,Mark D Long,Donald Kufe

Inflammation and regeneration 42:1 PubMed34983695

2022

Facilitation of colonic T cell immune responses is associated with an exacerbation of dextran sodium sulfate-induced colitis in mice lacking microsomal prostaglandin E synthase-1.

Applications

Unspecified application

Species

Unspecified reactive species

Fumiaki Kojima,Hiroki Sekiya,Yuka Hioki,Hitoshi Kashiwagi,Makoto Kubo,Masaki Nakamura,Shotaro Maehana,Yoshitaka Imamichi,Koh-Ichi Yuhki,Fumitaka Ushikubi,Hidero Kitasato,Takafumi Ichikawa

Mediators of inflammation 2021:5595520 PubMed33776573

2021

miR-708 Negatively Regulates TNF/IL-1 Signaling by Suppressing NF-B and Arachidonic Acid Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Monteleone,Carol S Lutz

Oncotarget 11:4699-4721 PubMed33473256

2020

miR-708-5p enhances erlotinib/paclitaxel efficacy and overcomes chemoresistance in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Monteleone,Carol S Lutz

Oncotarget 11:2464-2483 PubMed32655834

2020

miR-708-5p targets oncogenic prostaglandin E2 production to suppress a pro-tumorigenic phenotype in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Monteleone,Carol S Lutz

The Journal of biological chemistry 293:5295-5306 PubMed29440395

2018

Fatty acid-binding protein 5 controls microsomal prostaglandin E synthase 1 (mPGES-1) induction during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Diane Bogdan,Jerome Falcone,Martha P Kanjiya,Sang Hoon Park,Gregory Carbonetti,Keith Studholme,Maria Gomez,Yong Lu,Matthew W Elmes,Norbert Smietalo,Su Yan,Iwao Ojima,Michelino Puopolo,Martin Kaczocha

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765]

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (AB180589)

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (AB180589)

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (AB180589)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (10)

Product promise