AB248961

Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal PSB6 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free (AB248961)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free (AB248961)

This data was developed using ab150392, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling Proteasome 20S beta 6 with ab150392 at 1/100 dilution.

Unknown

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free (AB248961)

This data was developed using ab150392, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] (<a href='/ko/products/primary-antibodies/proteasome-20s-beta-6-antibody-epr9684-ab150392'>ab150392</a>) at 1/1000 dilution

Lane 1:

Human fetal liver cell lysate at 10 µg

Lane 2:

NCI-H460 cell lysate at 10 µg

Lane 3:

Caco 2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 25 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9684

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab248961 is the carrier-free version of ab150392.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S beta 6 also known as PSMB6 is a subunit of the proteasome complex. It plays a significant role in the protein degradation process. Its molecular weight is around 25 kDa. The subunit gets expressed in various cell types throughout the body ensuring that proteins are effectively broken down into peptides. This is essential for maintaining protein homeostasis and recycling faulty proteins.

Biological function summary

The proteasome 20S beta 6 subunit forms part of the 20S core complex an important component of the proteasome. The proteasome operates as the main proteolytic machinery in both nucleus and cytoplasm. The 20S core complex pairs with the regulatory 19S cap to form the 26S proteasome. This assembly degrades ubiquitin-tagged proteins which regulates cell cycle progression apoptosis and DNA repair contributing to cellular function and health.

Pathways

Proteasome 20S beta 6 acts within the ubiquitin-proteasome pathway. This pathway regulates protein turnover affecting critical cellular processes such as cell cycle apoptosis and the immune response. The pathway also links with other proteins such as ubiquitin and E1 E2 and E3 ligases which modify proteins with ubiquitin chains to signal for their degradation.

Proteasome 20S beta 6 may relate to cancer and neurodegenerative diseases like Alzheimer's. The abnormal regulation of the proteasome pathway can lead to the accumulation of undegraded proteins contributing to these diseases. PSMB6 interacts with proteins such as p53 a tumor suppressor protein highlighting its relevance to cell cycle control and cancer progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.

See full target information PSMB6

대체 명칭 보기

LMPY, Y, PSMB6, Proteasome subunit beta type-6, Macropain delta chain, Multicatalytic endopeptidase complex delta chain, Proteasome delta chain, Proteasome subunit Y, Proteasome subunit beta-1, beta-1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free (AB248961)

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] - BSA and Azide free (AB248961)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise