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AB184172

Anti-Proteasome 20S LMP2 antibody [EPR13785]

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(10 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Proteasome 20S LMP2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications.

대체 명칭 보기

LMP2, PSMB6i, RING12, PSMB9, Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein

4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)

Immunofluorescent analysis of 4% paraformaldehyde fixed HL-60 (Human promyelocytic leukemia cell line) cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution was used as secondary antibody. Counterstain : DAPI (blue).

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)
  • IP

Supplier Data

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)

Immunoprecipitation analysis of Raji cells labeling Proteasome 20S LMP2 using ab184172 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of Raji lysates. Blocking/dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

Predicted band size: 23 kDa

false

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)
  • WB

Lab

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Proteasome 20S LMP2 knockout HAP1 cell lysate (20 μg)
Lanes 1 - 2 : Merged signal (red and green). Green - ab184172 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab184172 was shown to specifically react with Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab184172 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

Predicted band size: 23 kDa

false

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)
  • WB

Supplier Data

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB184172)

All lanes:

Anti-EXTL1 antibody - C-terminal (<a href='/ko/products/unavailable/extl1-antibody-c-terminal-ab174172'>ab174172</a>) at 1/100000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Carrier free

    Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Proteasome 20S LMP2 antibody [EPR13785]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13785

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB3328

Anti-Proteasome 20S LMP2 antibody

  • 4
  • 5
Primary Antibodies

AB187645

Anti-Proteasome 20S LMP2 antibody [EPR13784]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

  • 0
  • 0
Primary Antibodies

AB243556

Anti-Proteasome 20S LMP2 antibody [EPR22042] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR22042] - BSA and Azide free (AB243556)

  • 0
  • 0
Primary Antibodies

AB242061

Anti-Proteasome 20S LMP2 antibody [EPR22042]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR22042] (AB242061)

  • 0
  • 0

보완 제품

Assay Kits

AB107921

Proteasome Activity Assay Kit

Functional Studies - Proteasome Activity Assay Kit (AB107921)

  • 4
  • 2
Primary Antibodies

AB183506

Anti-PSMB10/MECL1 antibody [EPR14902]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

  • 0
  • 0
Primary Antibodies

AB180606

Anti-Proteasome 20S LMP7 antibody [EPR14482(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

  • 5
  • 1
Primary Antibodies

AB75853

Anti-beta 2 Microglobulin antibody [EP2978Y]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] (AB75853)

  • 4
  • 9
Primary Antibodies

AB197054

Anti-Proteasome 26S S2/PSMD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 26S S2/PSMD2 antibody (AB197054)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The proteasome LMP2 also known as proteasome subunit beta type-1 or PSMB9 is a component important in the functioning of the 20S proteasome complex. This molecular entity with a mass of approximately 23 kDa is predominantly expressed in immune cells including lymphocytes. LMP2 incorporates into the immunoproteasome a specialized variant of the proteasome that participates prominently in the processing of class I MHC peptides enhancing the immune response.
Biological function summary

LMP2 is integral to protein catabolism and regulation of the cellular protein homeostasis. As part of the larger proteasome complex the LMP2 protein functions in degradative pathways that recycle amino acids from ubiquitin-tagged proteins. This proteasome takes part in antigen processing helping convert proteins into peptides that are presented on the surface of cells for immune system recognition.

Pathways

LMP2 is significantly involved in the ubiquitin-proteasome pathway and immune system processes. It interacts with other immunoproteasome subunits like LMP7 and MECL-1 which work together to enhance the proteolytic capacity for antigen presentation. The presence of LMP2 in these pathways demonstrates its role in adaptive immunity contributing to the clearance of misfolded or damaged proteins and therefore maintaining cell health.

LMP2 associates with immune-related conditions and some cancer types. Dysregulation of LMP2 expression or function can impact antigen presentation potentially leading to autoimmune disorders due to inappropriate immune activation. Moreover alterations in LMP2 expression have been observed in certain cancers such as melanoma where it affects proteasome activity and influences tumor progression. In these contexts interactions with proteins like TAP1 and TAP2 which aid in peptide transport mark its relevance in disease pathogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (PubMed : 33727065, PubMed : 34819510). The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
See full target information PSMB9
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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 23: PubMed36498987

2022

Immunoproteasome Inhibition Ameliorates Aged Dystrophic Mouse Muscle Environment.

Applications

Unspecified application

Species

Unspecified reactive species

Luana Tripodi,Davide Molinaro,Francesco Fortunato,Carolina Mella,Barbara Cassani,Yvan Torrente,Andrea Farini

Cell death & disease 13:975 PubMed36402750

2022

Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice.

Applications

Unspecified application

Species

Unspecified reactive species

A Farini,L Tripodi,C Villa,F Napolitano,F Strati,D Molinaro,F Facciotti,B Cassani,Y Torrente

American journal of cancer research 12:5286-5299 PubMed36504904

2022

Chronic stress promotes tumor immune evasion via the suppression of MHC-I expression and the upregulation of PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhu Chen,Yazhi Qian,Wei Huang,Yi Zhang,Mo Wu,Yinlong Cheng,Nan Yang,Yanyong Liu

Scientific reports 12:14087 PubMed35982220

2022

STUB1 is an intracellular checkpoint for interferon gamma sensing.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Ng,Shuhui Lim,Adrian Chong Nyi Sim,Ruban Mangadu,Ally Lau,Chunsheng Zhang,Sarah Bollinger Martinez,Arun Chandramohan,U-Ming Lim,Samantha Shu Wen Ho,Shih Chieh Chang,Pooja Gopal,Lewis Z Hong,Adam Schwaid,Aaron Zefrin Fernandis,Andrey Loboda,Cai Li,Uyen Phan,Brian Henry,Anthony W Partridge

Antioxidants (Basel, Switzerland) 11: PubMed35453413

2022

Hydroxysafflor Yellow A Blocks HIF-1 Induction of NOX2 and Protects ZO-1 Protein in Cerebral Microvascular Endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Xiao-Tian Liu,Pei-Lin Zhang,Yu-Chen Li,Meng-Ru Sun,Yi-Tao Wang,Sheng-Peng Wang,Hua Yang,Bao-Lin Liu,Mei Wang,Wen Gao,Ping Li

Cancer letters 529:37-52 PubMed34974132

2022

LC3 and NLRC5 interaction inhibits NLRC5-mediated MHC class I antigen presentation pathway in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhan,Junhui Zhang,Jing Zhang,Xiaojing Liu,Suding Zhu,Yuchuan Shi,Yu He,Wenyan Wang,Yijun Fan,Zhenhai Tang,Guo Chen,Bing Wei,Yunxia Cao

Nature communications 12:2099 PubMed33833239

2021

Defective dystrophic thymus determines degenerative changes in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Farini,Clementina Sitzia,Chiara Villa,Barbara Cassani,Luana Tripodi,Mariella Legato,Marzia Belicchi,Pamela Bella,Caterina Lonati,Stefano Gatti,Massimiliano Cerletti,Yvan Torrente

Journal of neuroinflammation 17:184 PubMed32532298

2020

The impact of regional astrocyte interferon-γ signaling during chronic autoimmunity: a novel role for the immunoproteasome.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon C Smith,Maksim Sinyuk,Julius E Jenkins,Morgan W Psenicka,Jessica L Williams

Frontiers in physiology 11:403 PubMed32508664

2020

PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Farini,Chiara Villa,Dario Di Silvestre,Pamela Bella,Luana Tripodi,Rossana Rossi,Clementina Sitzia,Stefano Gatti,Pierluigi Mauri,Yvan Torrente

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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