Anti-Proteasome Activator Subunit 4/PSME4 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (AB5620)

ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• WB

Supplier Data

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (AB5620)

A 200 kDa band corresponding to PSME4 was observed in HeLa, Jurkat, and mouse testis. In addition, a 140 kDa band corresponding to an isoform of PSME4 was observed across the cell lines and tissues tested.

Blocking - 5% skimmed milk

All lanes:

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/250 dilution

Lane 1:

HeLa whole cell extract at 30 µg

Lane 2:

Jurkat whole cell extract at 30 µg

Lane 3:

Mouse testis tissue extract at 30 µg

Lane 4:

Mouse pancreas tissue extract at 30 µg

Lane 5:

Rat pancreas tissue extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 211 kDa

false

• WB

Supplier Data

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (AB5620)

All lanes:

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution

All lanes:

Mouse testis tissue lysate at 25 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit

Predicted band size: 211 kDa

Observed band size: 160 kDa,200 kDa

true