AB137108

Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827]

Name: Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827]
Brand: Abcam Limited
SKU: AB137108
Rating: 5 (4 reviews)

RabMAb
Recombinant
각 태그의 뜻

(4 리뷰들)

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Proteasome subunit beta type 2/PSMB2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

대체 명칭 보기

Proteasome subunit beta type-2, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, Proteasome subunit beta-4, beta-4, PSMB2

2 이미지

Unknown

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (AB137108)

All lanes:

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 23 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (AB137108)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (AB137108)

Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labelling Proteasome subunit beta type 2/PSMB2 with ab137108 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8827

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit beta type 2 also known as PSMB2 is a part of the proteasome complex which plays an important role in protein degradation. PSMB2 has a mass of about 22 kDa and functions within the multi-catalytic proteinase complex known as the 20S proteasome core particle. This protein is expressed in various tissues with significant activities found in cells that demand high protein turnover. PSMB2 contributes to the proteasome's chymotrypsin-like activity aiding in the breakdown of damaged or unneeded proteins into peptides.

Biological function summary

Proteasome subunit beta type 2 is essential in maintaining protein homeostasis. It functions as a component of the 20S core of the 26S proteasome where it participates in degrading ubiquitin-tagged proteins. This complex also regulates multiple cellular processes including cell cycle progression and signal transduction. Proper function of PSMB2 is necessary for removing misfolded or damaged proteins thereby preventing their accumulation which could be toxic to cells.

Pathways

Proteasome subunit beta type 2 has a role in the ubiquitin-proteasome pathway which is important for protein degradation and turnover. This pathway contributes to important cellular processes such as DNA repair and antigen processing. PSMB2 in this context is linked to other proteins like ubiquitin-ligase that mediate the tagging of proteins for degradation. Another pathway involving PSMB2 is the NF-kB signaling pathway which controls inflammatory responses and immune system regulation.

Proteasome subunit beta type 2 has been implicated in neurodegenerative diseases like Parkinson’s. The dysregulation or malfunctioning of the proteasome complex might result in protein aggregation seen in such disorders. Additionally PSMB2 is associated with certain cancers due to its role in regulating apoptosis and cellular proliferative activities. Proteins like p53 which is important for tumor suppression are related to PSMB2 through these disease mechanisms highlighting its significance in maintaining normal cellular function and preventing disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMB2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2419607122 PubMed39964708

2025

Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina I Zittlau,Daniel Zachor-Movshovitz,Yegor Leushkin,Roy Schimmel Brener,David Morgenstern,Gili Ben-Nissan,Michal Sharon

EMBO reports 23:e54755 PubMed35642585

2022

Malaria parasites release vesicle subpopulations with signatures of different destinations.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Abou Karam,Irit Rosenhek-Goldian,Tamar Ziv,Hila Ben Ami Pilo,Ido Azuri,Anna Rivkin,Edo Kiper,Ron Rotkopf,Sidney R Cohen,Ana Claudia Torrecilhas,Ori Avinoam,Alicia Rojas,Mattia I Morandi,Neta Regev-Rudzki

Frontiers in endocrinology 13:882906 PubMed35574028

2022

Value of Immunohistochemical Expression of Apelin, Succinate Dehydrogenase B, Chromogranin B, Human Epidermal Growth Factor Receptor-2, Contactin 4, and Succinyl-CoA Synthetase Subunit Beta in Differentiating Metastatic From Non-Metastatic Pheochromocytoma and Paraganglioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Danlei Chen,Yingxian Pang,Xiaowen Xu,Xiao Guan,Longfei Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827]

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (AB137108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (AB137108)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise