AB244269

Anti-Protein CASP antibody

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Rabbit Polyclonal Protein CASP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CUX1 aa 50-250.

대체 명칭 보기

CUTL1, CUX1, Protein CASP

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody (AB244269)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody (AB244269)

PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Protein CASP (green) using ab244269 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Protein CASP using ab244269 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

Formalin-fixed, paraffin-embedded human Fallopian tube tissue stained for Protein CASP using ab244269 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for Protein CASP using ab244269 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Protein CASP antibody (AB244269)

All lanes:

Western blot - Anti-Protein CASP antibody (ab244269) at 0.4 µg/mL

All lanes:

Human thyroid glad tissue

Predicted band size: 77 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CUX1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q13948

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Protein CASP also known as Caspase or CASP protein is an important player in the process of apoptosis. Caspases are cysteine-aspartic proteases that exist as inactive proenzymes made of a prodomain large and small subunits. Upon activation caspases cleave substrates after aspartic acid residues. The Caspase family proteins have similar structures with molecular masses around 20 kDa to 40 kDa. CASP proteins are widely expressed across different tissues in the body. Activation can happen through intrinsic and extrinsic pathways enabling them to mediate the orderly dismantling of cellular components.

Biological function summary

Protein CASP acts as an executioner enzyme in cell death. It forms part of intricate protein complexes notably the apoptosome which includes APAF1 and cytochrome c. Once activated CASP proteins initiate a cascade of proteolytic events that lead to the dismantling of the cell. This process is tightly regulated and is essential for maintaining homeostasis and tissue development. Other caspases work upstream to activate downstream executioner caspases further highlighting the protein's role in these biological functions.

Pathways

Protein CASP plays a significant role in apoptotic signaling pathways. It is pivotal in both the extrinsic and intrinsic apoptotic pathways. In the extrinsic pathway proteins such as FADD and DISC interact with CASP-8 to trigger apoptosis. The intrinsic pathway includes the release of cytochrome c which partners with APAF1 leading to the activation of CASP-9 and subsequently other caspases. This cascade illustrates the interconnected nature of apoptotic signaling with other molecular pathways ensuring the precise regulation of cell death.

Protein CASP is associated with cancer and neurodegenerative diseases. Dysregulation of CASP activity can result in either excessive cell death or survival contributing to disease pathogenesis. In cancers often CASP activity is impaired allowing cancer cells to evade apoptosis and proliferate unchecked. In neurodegenerative disorders such as Alzheimer's abnormal CASP activation contributes to neuronal loss. The interaction between CASP proteins and BCL-2 family members highlights their role in these diseases emphasizing the therapeutic targeting potential in modulating CASP activity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May be involved in intra-Golgi retrograde transport.

See full target information CUX1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in molecular neuroscience 16:1083850 PubMed37033374

2023

Involvement of endoplasmic reticulum stress in trigeminal ganglion corneal neuron injury in dry eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Zhang,Hongbin Lin,Fengxian Li,Kaili Wu,Shuangjian Yang,Shiyou Zhou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Protein CASP antibody

Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody (AB244269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody (AB244269)

Western blot - Anti-Protein CASP antibody (AB244269)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise