Anti-PRPF8/Prp8 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (AB245504)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRPF8/Prp8 using ab245504 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining.

• IP

Supplier Data

Immunoprecipitation - Anti-PRPF8/Prp8 antibody (AB245504)

PRPF8/Prp8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245504 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245504 at 1 μg/ml.

Lane 1 : ab245504 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PRPF8/Prp8 antibody (ab245504)

Predicted band size: 274 kDa

false

• WB

Supplier Data

Western blot - Anti-PRPF8/Prp8 antibody (AB245504)

All lanes:

Western blot - Anti-PRPF8/Prp8 antibody (ab245504) at 0.1 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 274 kDa

true

Exposure time: 10s