Anti-PRUNE antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRUNE antibody (AB237518)

Paraffin-embedded human lung tissue stained for PRUNE using ab237518 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PRUNE antibody (AB237518)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PRUNE (green) using ab237518 at 1/135 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRUNE antibody (AB237518)

Paraffin-embedded human small intestine tissue stained for PRUNE using ab237518 at 1/100 dilution in immunohistochemical analysis.