AB221645

Anti-PSMD11 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal PSMD11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSMD11 aa 1-100.

대체 명칭 보기

26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p44.5, PSMD11

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-PSMD11 antibody (AB221645)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSMD11 antibody (AB221645)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PSMD11 (green) using ab221645 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD11 antibody (AB221645)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD11 antibody (AB221645)

Paraffin-embedded human testis tissue stained for PSMD11 using ab221645 at 1/50 dilution in Immunohistochemical analysis.

Supplier Data

Western blot - Anti-PSMD11 antibody (AB221645)

All lanes:

Western blot - Anti-PSMD11 antibody (ab221645) at 1/100 dilution

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate.

Predicted band size: 47 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PSMD11 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O00231

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PSMD11 also known as RPN-6 is a regulatory subunit of the proteasome 26S. It is approximately 50 kDa in mass and plays an essential role in the proteasome's function responsible for protein degradation within cells. PSMD11 is broadly expressed in various tissues including high expression in the brain heart and skeletal muscle. Its mechanical role involves binding to other subunits to stabilize the proteasome structure and facilitate substrate entry into the proteolytic core.

Biological function summary

PSMD11 is essential for the proper assembly and activity of the 26S proteasome complex which is important for ubiquitin-mediated proteolysis. This process controls protein turnover and regulates the concentration of various proteins to maintain cellular homeostasis. PSMD11 enhances the degradation of misfolded or damaged proteins therefore preventing the accumulation of toxic protein aggregates within cells. Its expression levels can modulate the proteasome activity in response to cellular conditions.

Pathways

PSMD11 plays a significant role in the ubiquitin-proteasome pathway which is critical for protein homeostasis and degradation of regulatory proteins. This pathway involves several important proteins including ubiquitin which tags substrates for degradation. PSMD11 also interfaces with signaling pathways that regulate cell cycle progression and apoptosis such as interactions with p53 and c-Myc proteins. These pathways ensure the balance between cell survival and programmed cell death.

PSMD11 links directly to conditions involving proteasome dysfunction such as neurodegenerative diseases including Parkinson's disease and Alzheimer's disease. Dysregulation of PSMD11 can impact proteasome activity leading to the buildup of unwanted proteins that contribute to these conditions. Additionally PSMD11's interaction with proteins like alpha-synuclein and tau in these diseases highlights its relevance in understanding their pathogenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs) : its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

See full target information PSMD11

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of thoracic disease 16:7819-7835 PubMed39678883

2024

Prognostic value and potential biological function of in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Xi,Jing Zeng,Yundong Zhou,Weiyu Shen,Hirokazu Taniguchi,Retnagowri Rajandram,Sivakumar Krishnasamy

Cell reports 26:1303-1317.e7 PubMed30699356

2019

Multivalent Role of Human TFIID in Recruiting Elongation Components at the Promoter-Proximal Region for Transcriptional Control.

Applications

Unspecified application

Species

Unspecified reactive species

Dipika Yadav,Koushik Ghosh,Subham Basu,Robert G Roeder,Debabrata Biswas

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMD11 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PSMD11 antibody (AB221645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD11 antibody (AB221645)

Western blot - Anti-PSMD11 antibody (AB221645)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise