Anti-PSME1 antibody [EPR10967(B)]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

• I-ELISA

Lab

Indirect ELISA - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Indirect ELISA analysis of Human Proteasome activator complex subunit 1 using ab155091 at 1000 ng/mL dilution and secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 2500 ng/mL dilution

• WB

Lab

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Western blot : Anti-PSME1 antibody [EPR10967(B)] ab155091 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 29 kDa in Wild-type HEK-293T cell lysates with no signal observed at this size in PSME1 knockout HEK-293T cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysates at 20 µg

Lane 2:

Western blot - Human PPM1G knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-ppm1g-knockout-hek-293t-cell-line-ab266162'>ab266162</a>) at 20 µg

Lane 3:

MCF7 cell lysates at 20 µg

Lane 4:

Jurkat cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Unknown

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 29 kDa

false

• WB

AbReview40987****

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

All lanes:

Human prostate cancer whole tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview