Anti-PSPH antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSPH antibody (AB224110)

Paraffin-embedded human skeletal muscle tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis. No positivity observed.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSPH antibody (AB224110)

Paraffin-embedded human Fallopian tube tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSPH antibody (AB224110)

Paraffin-embedded human kidney tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSPH antibody (AB224110)

Paraffin-embedded human endometrium tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-PSPH antibody (AB224110)

Western blot analysis labeling PSPH with ab224110 at 0.04 ug/ml.

All lanes:

Western blot - Anti-PSPH antibody (ab224110) at 0.04 µg/mL

Lane 1:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 25 kDa

false

• WB

Lab

Western blot - Anti-PSPH antibody (AB224110)

Western blot : Anti-PSPH antibody (ab224110) staining at 0.1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab224110 was shown to bind specifically to PSPH. A band was observed at 25 kDa in wild-type A549 cell lysates with no signal observed at this size in PSPH knockout cell line. To generate this image, wild-type and PSPH knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PSPH antibody (ab224110) at 0.1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

PSPH knockout A549 cell lysate at 20 µg

Lane 3:

Caco-2 cell lysate at 20 µg

Lane 4:

A549 Nuclear cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 25 kDa

false