AB238338

Anti-PTCH2 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal PTCH2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTCH2 aa 750-1000.

대체 명칭 보기

UNQ560/PRO1121/PRO57079, PTCH2, Protein patched homolog 2, PTC2

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody (AB238338)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody (AB238338)

HEK-293 (Human epithelial cell line from embryonic kidney) cells stained for PTCH2 (green) using ab238338 at 1/66 dilution in ICC/IF. Secondary antibody is an Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody (AB238338)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody (AB238338)

Paraffin-embedded human brain tissue stained for PTCH2 using ab238338 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PTCH2 aa 750-1000. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6C5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PTCH2 also known as Patched 2 is a protein with a mass of approximately 145 kDa. It is a member of the Patched family of proteins and functions as a transmembrane receptor. PTCH2 is predominantly found on the surface of cells in tissues such as skin brain and testes. The protein serves an important role in regulating the signaling of the Hedgehog pathway by binding to its ligand Sonic Hedgehog which controls the activity of the smoothened (SMO) receptor.

Biological function summary

PTCH2 participates in the negative regulation of the Hedgehog signaling pathway. It forms a receptor complex with other proteins to maintain proper cell communication and growth control. PTCH2 is part of a larger complex that includes Patched 1 (PTCH1) contributing to embryonic development and tissue patterning. Its regulation of cellular growth and differentiation processes is critical for these activities.

Pathways

PTCH2 is a critical component of the Hedgehog signaling pathway and the development pathway. It closely interacts with PTCH1 and SMO in regulating signal transduction. Communication within these pathways is essential for tissue patterning and organogenesis. PTCH2's modulation of SMO results in downstream effects which impact the transcription of target genes controlling cell proliferation.

PTCH2 has a link to basal cell carcinoma and medulloblastoma. Its aberrant signaling and mutations may lead to uncontrolled cellular proliferation contributing to the onset of these cancers. The protein's interaction with PTCH1 also affects cancer susceptibility since both proteins can influence the progression and risk of developing tumors associated with the Hedgehog pathway.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the control of cellular growth (PubMed : 18285427). May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH).

See full target information PTCH2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 29:15 PubMed39492940

2024

UL16‑binding protein 1 is a significant prognostic and diagnostic marker for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Zhang,Shuhong Dai,Liang Li,Pengyun Wang,Mingxin Dong

PloS one 14:e0225511 PubMed31756206

2019

Expression profile of sonic hedgehog signaling-related molecules in basal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Sung Kim,Young Sil Kim,Chul Lee,Myung Soo Shin,Jae Wang Kim,Bo Gun Jang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTCH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody (AB238338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody (AB238338)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise